Rabbit PALB2 Polyclonal Antibody | anti-PALB2 antibody

PALB2 Antibody

Cat. Num. AAA150755

• Gene Names: PALB2; FANCN; PNCA3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: PALB2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PALB2; Polyclonal Antibody; PALB2 Antibody; FANCN; PNCA3; Partner and localizer of BRCA2; partner and localizer of BRCA2; anti-PALB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PALB2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1186

• Applicable Applications for anti-PALB2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: PALB2 antibody can be used for detection of PALB2 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PALB2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human PALB2.
• Buffer: PALB2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PALB2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PALB2 in SK-N-SH cell lysate with PALB2 antibody at 1 μg/mL.)

Related Product Information for anti-PALB2 antibody

PALB2 Antibody: The breast cancer early onset proteins BRCA1 and BRCA2 are central to the repair of DNA damage by homologous recombination and are strongly associated with inherited breast and ovarian cancer. The protein PALB2 is required for the localization of BRCA2 to sites of DNA damage and like BRCA1 and 2 is a breast cancer susceptibility gene. PALB2 is thought to function through directly binding to BRCA1, which allows the PALB2 protein to organize BRCA2 and the recombinase RAD51 at the site of DNA damage. Analysis of the BRCA1-PALB2-BRCA2-RAD51network may allow predictions to be made of the responsiveness of a particular tumor to therapeutic treatments. At least four isoforms of PALB2 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 74727919
• NCBI GeneID: 79728
• UniProt Accession #: Q86YC2; Q8N7Y6; Q8ND31; Q9H6W1; A6NIE1
• Molecular Weight: 131,295 Da
• NCBI Official Full Name: Partner and localizer of BRCA2
• NCBI Official Synonym Full Names: partner and localizer of BRCA2
• NCBI Official Symbol: PALB2
• NCBI Official Synonym Symbols: FANCN; PNCA3
• NCBI Protein Information: partner and localizer of BRCA2
• UniProt Protein Name: Partner and localizer of BRCA2
• Protein Family: Partner and localizer of BRCA2
• UniProt Gene Name: PALB2
• UniProt Synonym Gene Names: FANCN
• UniProt Entry Name: PALB2_HUMAN

NCBI Description

This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2. [provided by RefSeq, Jul 2008]

Uniprot Description

PALB2: Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. Defects in PALB2 are a cause of susceptibility to breast cancer (BC). A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Breast cancer susceptibility is strongly associated with PALB2 truncating mutations. Conversely, rare missense mutations do not strongly influence breast cancer risk (PubMed:22241545). Defects in PALB2 are the cause of Fanconi anemia complementation group N (FANCN). It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. Defects in PALB2 are the cause of pancreatic cancer type 3 (PNCA3). It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding

Biological Process: somitogenesis; organ morphogenesis; mesoderm development; DNA repair; inner cell mass cell proliferation; double-strand break repair via homologous recombination; negative regulation of apoptosis

Disease: Pancreatic Cancer, Susceptibility To, 3; Breast Cancer; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group N