Rabbit PAK4 Polyclonal Antibody | anti-PAK4 antibody

PAK4 Rabbit Polyclonal

Cat. Num. AAA7602356

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
• Purity: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen Affinity Purified
• Synonyms: PAK4; Polyclonal Antibody; PAK4 Rabbit Polyclonal; KIAA1142; p21 activated kinase 4; PAK 4; anti-PAK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology
• Purity/Purification: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 591

• Applicable Applications for anti-PAK4 antibody: ELISA, WB, IHC, IF, FC, IP
• Application Notes: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
• Immunogen: p21 protein(Cdc42/Rac)-activated kinase 4
• Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
• Preparation and Storage: -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7602356 (PAK4 antibody) at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells, using PAK4 antibody MBS7602356 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)

Immunoprecipitation (IP)

(IP Result of anti-PAK4 (IP: MBS7602356, 4ug; Detection: MBS7602356 1:1000) with mouse liver tissue lysate 3000ug.)

Western Blot (WB)

(COLO 320 cells were subjected to SDS PAGE followed by western blot with MBS7602356 (PAK4 antibody) at dilution of 1:800)

Related Product Information for anti-PAK4 antibody

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

NCBI and Uniprot Product Information

• NCBI GI #: 4164385
• UniProt Accession #: O96013; Q8N4E1; Q8NCH5; Q8NDE3; Q9BU33; Q9ULS8; B4DGG6
• Molecular Weight: 64 kDa
• NCBI Official Full Name: PAK4 protein
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 4
• NCBI Official Symbol: PAK4
• NCBI Protein Information: serine/threonine-protein kinase PAK 4
• UniProt Protein Name: Serine/threonine-protein kinase PAK 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK4
• UniProt Synonym Gene Names: KIAA1142; PAK-4
• UniProt Entry Name: PAK4_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: focal adhesion; Golgi apparatus

Molecular Function: ATP binding; protein binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; cell growth; cell migration; cell motility; cell proliferation; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction