Rabbit anti-Human PAK4 Polyclonal Antibody | anti-PAK4 antibody

PAK4 Antibody

Cat. Num. AAA150320

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PAK4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PAK4; Polyclonal Antibody; PAK4 Antibody; KIAA1142; p21-activated kinase 4; PAK-4; p21 protein (Cdc42/Rac)-activated kinase 4; anti-PAK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PAK4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 591

• Applicable Applications for anti-PAK4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4.
• Buffer: PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PAK4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 μg/mL.)

Related Product Information for anti-PAK4 antibody

PAK4 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 5031975
• NCBI GeneID: 10298
• NCBI Accession #: NP_005875
• NCBI GenBank Nucleotide #: NM_005884.3
• UniProt Accession #: O96013; Q8N4E1; Q8NCH5; Q8NDE3; Q9BU33; Q9ULS8; B4DGG6
• Molecular Weight: 54,940 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 4 isoform 1
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 4
• NCBI Official Symbol: PAK4
• NCBI Protein Information: serine/threonine-protein kinase PAK 4; PAK-4; p21-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• UniProt Protein Name: Serine/threonine-protein kinase PAK 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK4
• UniProt Synonym Gene Names: KIAA1142; PAK-4
• UniProt Entry Name: PAK4_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: Golgi apparatus; focal adhesion

Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: cell proliferation; cell migration; apoptosis; cytoskeleton organization and biogenesis; mitotic cell cycle; cell motility; signal transduction; cell growth; protein amino acid phosphorylation