Western Blot (WB)
(Western blot analysis of lysates from Hela, Jurkat cell line (from left to right), using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit Pak3 Polyclonal Antibody | anti-Pak3 antibody
Mouse Pak3 Antibody (N-term)
Predicted Reactivity: Human, Rat, Xenopus
Predicted Reactivity: Human, Rat, Xenopus
IHC-P~~1:10~50
Western Blot (WB)
(Western blot analysis of lysates from Hela, Jurkat cell line (from left to right), using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from Hela, Jurkat cell line (from left to right), using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Pak3 Antibody (N-term). MBS9209950 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Immunohistochemistry (IHC)
(Mouse Pak3 Antibody (N-term) (MBS9209950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Pak3 Antibody (N-term) (MBS9209950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Pak3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Jiang, X.S., et al. Hum. Mol. Genet. 19(7):1347-1357(2010)
Demyanenko, G.P., et al. Neuroscience 165(1):107-115(2010)
Grimsley-Myers, C.M., et al. J. Neurosci. 29(50):15859-15869(2009)
Sansom, S.N., et al. PLoS Genet. 5 (6), E1000511 (2009) :
NCBI and Uniprot Product Information
Uniprot Description
PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE20 family; PAKA subfamily
Cellular Component: cytoplasm; endosome
Molecular Function: Rho GTPase binding; metal ion binding; nucleotide binding; protein kinase activity; transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; GTPase binding; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; SH3 domain binding; catalytic activity; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: regulation of actin filament polymerization; axonogenesis; activation of MAPK activity; metabolic process; multicellular organismal development; positive regulation of neuron apoptosis; MAPKKK cascade; dendrite development; response to stress; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation; phosphorylation
Research Articles on Pak3
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Product Notes
The Pak3 pak3 (Catalog #AAA9209950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 124-152. The Mouse Pak3 Antibody (N-term) reacts with Mouse Predicted Reactivity: Human, Rat, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Pak3 can be used in a range of immunoassay formats including, but not limited to, WB, IHC-P,E. WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the Pak3 pak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pak3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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