Western Blot (WB)
(Western blot analysis of PAK3(arrow) using rabbit polyclonal PAK3 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human PAK3 Polyclonal Antibody | anti-Pak3 antibody
PAK3 (p21 Activated Kinase 3, p21-activated Kinase 3, p21(CDKN1A)-activated Kinase 3, PAK-3, PAK3beta, Beta PAK, Beta-PAK, bPAK, CDKN1A, hPAK3, Mental Retardation X-linked 30, MRX30, MRX47, Oligophrenin 3, OPHN3, Serine/threonine Protein Kinase PAK 3)
Gene Names
Pak3; anon-WO0118547.285; CG14895; D-Pak3; DmelCG14895; DmPAK3; dPAK3; DPak3; DPAK3; pak3; PaK3; PAK3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
PAK3; Polyclonal Antibody; PAK3 (p21 Activated Kinase 3; p21-activated Kinase 3; p21(CDKN1A)-activated Kinase 3; PAK-3; PAK3beta; Beta PAK; Beta-PAK; bPAK; CDKN1A; hPAK3; Mental Retardation X-linked 30; MRX30; MRX47; Oligophrenin 3; OPHN3; Serine/threonine Protein Kinase PAK 3); Anti -PAK3 (p21 Activated Kinase 3; anti-Pak3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PAK3.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-Pak3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunogen
Synthetic peptide selected from the central region of human PAK3 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PAK3(arrow) using rabbit polyclonal PAK3 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PAK3(arrow) using rabbit polyclonal PAK3 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-Pak3 antibody
PAK3, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. PAK3 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. It shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK. This protein is highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. PAK3 is autophosphorylated when activated by CDC42/p21. Defects in PAK3 are the cause of non-specific X-linked nonsyndromic mental retardation type 30 (MRX30). The protein structure contains 1 CRIB domain.
Product Categories/Family for anti-Pak3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,146 Da
NCBI Official Full Name
Pak3, isoform D
NCBI Official Symbol
Pak3
NCBI Official Synonym Symbols
anon-WO0118547.285; CG14895; D-Pak3; DmelCG14895; DmPAK3; dPAK3; DPak3; DPAK3; pak3; PaK3; PAK3
NCBI Protein Information
CG14895 gene product from transcript CG14895-RD; CG14895-PA; CG14895-PC; CG14895-PD; Pak3; Pak3-PA; Pak3-PC; Pak3-PD
UniProt Protein Name
LD10376p
Protein Family
UniProt Gene Name
Pak3
UniProt Entry Name
Q9VEV1_DROME
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein. SAAS SAAS000095
Sequence similarities: Contains CRIB domain. SAAS SAAS000095Contains protein kinase domain. SAAS SAAS000095
Research Articles on Pak3
Similar Products
Product Notes
The Pak3 pak3 (Catalog #AAA623880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAK3 (p21 Activated Kinase 3, p21-activated Kinase 3, p21(CDKN1A)-activated Kinase 3, PAK-3, PAK3beta, Beta PAK, Beta-PAK, bPAK, CDKN1A, hPAK3, Mental Retardation X-linked 30, MRX30, MRX47, Oligophrenin 3, OPHN3, Serine/threonine Protein Kinase PAK 3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the Pak3 pak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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