Rabbit anti-Human PAK1 Polyclonal Antibody | anti-PAK1 antibody

PAK1, Phosphorylated (Thr423) (Serine/Threonine-protein Kinase PAK 1, p21-activated Kinase 1, PAK-1, p65-PAK, Alpha-PAK) (APC)

Cat. Num. AAA6491780

• Gene Names: PAK1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PAK1; Polyclonal Antibody; Phosphorylated (Thr423) (Serine/Threonine-protein Kinase PAK 1; p21-activated Kinase 1; PAK-1; p65-PAK; Alpha-PAK) (APC); anti-PAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAK1 when phosphorylated at Thr423.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 545

• Applicable Applications for anti-PAK1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr423 of human PAK1 (KLH).
• Conjugate: APC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Phospho-PAK1-pT423 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Dot Blot (DB)

(Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-PAK1 antibody

Antibodies; Protein Kinases

References

1. Li, W., et al., J. Biol. Chem. 279(31):32824-32831 (2004). 2. DerMardirossian, C., et al., Mol. Cell 15(1):117-127 (2004). 3. Weber, D.S., et al., Circ. Res. 94(9):1219-1226 (2004). 4. Balasenthil, S., et al., J. Biol. Chem. 279(2):1422-1428 (2004). 5. Wu, R.F., et al., J. Virol. 78(2):779-789 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 42794769
• NCBI GeneID: 5058
• NCBI Accession #: NP_002567
• NCBI GenBank Nucleotide #: NP_002567.3
• UniProt Accession #: Q13153
• Molecular Weight: 60.516
• NCBI Official Full Name: serine/threonine-protein kinase PAK 1 isoform 2
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 1
• NCBI Official Symbol: PAK1
• NCBI Official Synonym Symbols: IDDMSSD; p65-PAK; PAKalpha; alpha-PAK
• NCBI Protein Information: serine/threonine-protein kinase PAK 1
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK1
• UniProt Synonym Gene Names: PAK-1
• UniProt Entry Name: PAK1_HUMAN

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK.

Protein type: EC 2.7.11.1; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 11q13-q14

Cellular Component: Golgi apparatus; nuclear membrane; focal adhesion; protein complex; dendrite; intercellular junction; Z disc; cytosol; filamentous actin; ruffle; growth cone; axon; cytoplasm; plasma membrane

Molecular Function: collagen binding; protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; exocytosis; wound healing; apoptosis; protein amino acid autophosphorylation; positive regulation of estrogen receptor signaling pathway; receptor clustering; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; dendrite development; neuromuscular junction development; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cellular response to insulin stimulus; branching morphogenesis of a tube; actin cytoskeleton reorganization; T cell costimulation; response to hypoxia; innate immune response; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; neurite morphogenesis