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Testing Data

Rabbit anti-Mouse, human PAK-1 (Thr84) Polyclonal Antibody | anti-PAK1 antibody

Anti-Phospho-Thr84 PAK1

Gene Names
PAK1; PAKalpha
Reactivity
Mouse, human
Applications
Western Blot
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
Synonyms
PAK-1 (Thr84); Polyclonal Antibody; Anti-Phospho-Thr84 PAK1; anti-PAK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, human
Clonality
Polyclonal
Specificity
Specific for the ~68k PAK1 phosphorylated at Thr84.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Sequence Length
553
Applicable Applications for anti-PAK1 antibody
Western Blot (WB)
Application Notes
Quality Control: Western blots performed on each lot.
WB: 1:1000
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr84 of human PAK1.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, chicken, non-human primates, rat, sheep and zebra fish
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with mouse and human tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, non-human primates, rat, sheep, and zebrafish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin (Chen et al, 2004).
Preparation and Storage
Store at -20 degree C; stable for at least 1 year

Testing Data

Testing Data
Related Product Information for anti-PAK1 antibody
Affinity purified rabbit polyclonal antibody
References
• Chen W, Yazicioglu M, Cobb MH (2004) Characterization of OSR1, a member of the mammalian Ste20p/germinal center kinase family. J of Biol Chem 279 (12):11129-36.
• Ye D, Field J (2012) PAK signaling in cancer. Cell Logist 2(2):105-116.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68
NCBI Official Full Name
serine/threonine-protein kinase PAK 1 isoform 1
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 1
NCBI Official Symbol
PAK1
NCBI Official Synonym Symbols
PAKalpha
NCBI Protein Information
serine/threonine-protein kinase PAK 1; p65-PAK; alpha-PAK; STE20 homolog, yeast; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
UniProt Protein Name
Serine/threonine-protein kinase PAK 1
UniProt Gene Name
PAK1
UniProt Synonym Gene Names
PAK-1
UniProt Entry Name
PAK1_HUMAN

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Ref.1 Ref.2 Ref.6 Ref.8 Ref.10 Ref.12 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.22 Ref.23 Ref.24 Ref.32 Ref.34

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.6 Ref.10 Ref.38

Cofactor: Magnesium.

Enzyme regulation: Phosphorylation of Thr-84 by OXSR1 inhibits activation

By similarity. Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, and enables activation by phosphorylation of Thr-423. Ref.6 Ref.11 Ref.13 Ref.37

Subunit structure: Homodimer in its autoinhibited state. Active as monomer. Component of cytoplasmic complexes, which also contains PXN, ARHGEF6 and GIT1. Interacts with NISCH

By similarity. Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering

By similarity. Binds to the caspase-cleaved p110 isoform ofCDC2L1 and CDC2L2, p110C, but not the full-length proteins. Interacts with ARHGEF7. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interacts with NCK1 and NCK2. Interacts with TBCB. Interacts with CRIPAK. Interacts with BRSK2. Interacts with SNAI1. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.20 Ref.22 Ref.25 Ref.26 Ref.32 Ref.36 Ref.38

Subcellular location: Cytoplasm. Cell junction › focal adhesion. Cell membrane. Note: Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Ref.6 Ref.16

Post-translational modification: Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites. Ref.6 Ref.10 Ref.11 Ref.16 Ref.23 Ref.24 Ref.32 Ref.34 Ref.38

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.

Research Articles on PAK1

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Product Notes

The PAK1 pak1 (Catalog #AAA502190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Thr84 PAK1 reacts with Mouse, human and may cross-react with other species as described in the data sheet. AAA Biotech's PAK-1 (Thr84) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Quality Control: Western blots performed on each lot. WB: 1:1000. Researchers should empirically determine the suitability of the PAK1 pak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK-1 (Thr84), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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