Rabbit Paip2A Polyclonal Antibody | anti-PAIP2 antibody

Paip2A (PABP Binding Protein 2A)

Cat. Num. AAA623149

• Gene Names: PAIP2; PAIP2A
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Paip2A; Polyclonal Antibody; Paip2A (PABP Binding Protein 2A); Anti -Paip2A (PABP Binding Protein 2A); anti-PAIP2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Paip2A. Species crossreactivity: mouse, rat, monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in10mM sodium HEPES pH 7.5, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-PAIP2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic peptide derived from human Paip2A (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from mouse testis and human testis using MBS623149.)

Related Product Information for anti-PAIP2 antibody

The eukaryotic mRNA 3' poly(A) tail interacts with the 5' cap structure to regulate translation of mRNA transcripts (1). The eIF4G translation initiation factor associates with poly(A) binding protein (PABP) to circularize mRNA (1,2). Paip1 is a PABP interacting protein that binds eIF4A to stimulate translation (3). A second PABP binding protein, Paip2, inhibits translation by interfering with PABP function. Specifically, Paip2 reduces the affinity of PABP for poly(A) RNA and competes with Paip1 for PABP binding (1,2).

Product Categories/Family for anti-PAIP2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 75677355
• NCBI GeneID: 51247
• NCBI Accession #: NP_001028284.1
• NCBI GenBank Nucleotide #: NM_001033112.1
• UniProt Accession #: Q9BPZ3; Q49A06; Q9H0Y5; Q9P0Q8; B2RBI1; D3DQC6
• Molecular Weight: 14,984 Da
• NCBI Official Full Name: polyadenylate-binding protein-interacting protein 2
• NCBI Official Synonym Full Names: poly(A) binding protein interacting protein 2
• NCBI Official Symbol: PAIP2
• NCBI Official Synonym Symbols: PAIP2A
• NCBI Protein Information: polyadenylate-binding protein-interacting protein 2; PAIP-2; PABP-interacting protein 2; PABC1-interacting protein 2; polyA-binding protein-interacting protein 2
• UniProt Protein Name: Polyadenylate-binding protein-interacting protein 2
• Protein Family: Polyadenylate-binding protein-interacting protein
• UniProt Gene Name: PAIP2
• UniProt Synonym Gene Names: PAIP2A; PABP-interacting protein 2; PAIP-2
• UniProt Entry Name: PAIP2_HUMAN

Uniprot Description

PAIP2: Acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization. Interacts with the second and third RRM domains and C- terminus regions of PABPC1 in a 2:1 stoichiometry. Belongs to the PAIP2 family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoplasm

Molecular Function: mRNA binding; protein binding; translation repressor activity

Biological Process: negative regulation of translational initiation; spermatogenesis; memory