Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PAI1/SERPINE1 Polyclonal Antibody | anti-SERPINE1 antibody
Goat anti-PAI1/SERPINE1 Antibody
Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration, 4-6ug/ml. A customer successfully used this product on Human Breast cancer.
Western Blot: Approx 48kDa band observed in Human Liver, Lung and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
Testing Data
((1.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
Testing Data
((1.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: extracellular matrix; extracellular region; extracellular space; plasma membrane
Molecular Function: protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity
Biological Process: angiogenesis; chronological cell aging; circadian rhythm; defense response to Gram-negative bacterium; extracellular matrix organization and biogenesis; fibrinolysis; negative regulation of blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of smooth muscle cell migration; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of receptor-mediated endocytosis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity
Disease: Plasminogen Activator Inhibitor-1 Deficiency
Research Articles on SERPINE1
Similar Products
Product Notes
The SERPINE1 serpine1 (Catalog #AAA420848) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PAI1/SERPINE1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PAI1/SERPINE1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration, 4-6ug/ml. A customer successfully used this product on Human Breast cancer. Western Blot: Approx 48kDa band observed in Human Liver, Lung and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml. Researchers should empirically determine the suitability of the SERPINE1 serpine1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GFKIDDKGMA PALRH. It is sometimes possible for the material contained within the vial of "PAI1/SERPINE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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