anti-Human PAI-3 Polyclonal Antibody | anti-PAI-3 antibody

PAI-3 Antibody

Cat. Num. AAA8527288

• Gene Names: SERPINA5; PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity purified
• Synonyms: PAI-3; Polyclonal Antibody; PAI-3 Antibody; anti-PAI-3 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human PAI3.
• Purity/Purification: Affinity purified
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
• Sequence Length: 406

• Applicable Applications for anti-PAI-3 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:200
• Preservative: 0.05% Sodium Azide.
• Cellular Localization: Secretory Protein
• Positive Control: Human Liver
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-PAI-3 antibody

PAI-3 also called Protein C inhibitor (PCI) was originally described in plasma as an inhibitor of the anticoagulant serine protease activated protein C. PCI is present not only in plasma, but also in urine and in many other body fluids and secretions. The highest PCI concentrations have been described in seminal plasma (?200 mug/ml). The inhibition is regulated by glycosaminoglycans, such as heparin and heparan sulphate, and several polyanions. As well as its roles in the regulation of hemostasis and fertilisation, Protein C Inhibitor is involved in cell inflammation, proliferation, apoptosis, tumor cell migration, invasion, and metastasis.

References

1. "Protein C inhibitor from human plasma: characterization of native and cleaved inhibitor and demonstration of inhibitor complexes with plasma kallikrein."Laurell M., Stenflo J.Thromb. Haemost. 62:885-891(1989). 2. "A two-allele polymorphism in protein C inhibitor with varying frequencies in different ethnic populations."Radtke K.-P., Greengard J.S., Fernandez J.A., Villoutreix B.O., Griffin J.H.Thromb. Haemost. 75:62-69(1996). 3. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.J. Proteome Res. 4:2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 194018472
• NCBI GeneID: 5104
• NCBI Accession #: NP_000615.3
• NCBI GenBank Nucleotide #: NM_000624.5
• UniProt Accession #: P05154; Q07616; Q9UG30
• Molecular Weight: 46kDa
• NCBI Official Full Name: plasma serine protease inhibitor preproprotein
• NCBI Official Synonym Full Names: serpin family A member 5
• NCBI Official Symbol: SERPINA5
• NCBI Official Synonym Symbols: PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3
• NCBI Protein Information: plasma serine protease inhibitor
• UniProt Protein Name: Plasma serine protease inhibitor
• UniProt Gene Name: SERPINA5
• UniProt Synonym Gene Names: PCI; PLANH3; PROCI; PAI-3; PAI3; PCI

NCBI Description

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. [provided by RefSeq, Aug 2012]

Uniprot Description

Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.