Sheep PAI-1 - Plasminogen Activator Inhibitor type 1 Polyclonal Antibody | anti-SERPINE1 antibody

Sheep anti-human Plasminogen Activator Inhibitor Type 1, Peroxidase Conjugated IgG

Cat. Num. AAA512113

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Purity: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Synonyms: PAI-1 - Plasminogen Activator Inhibitor type 1; Polyclonal Antibody; Sheep anti-human Plasminogen Activator Inhibitor Type 1; Peroxidase Conjugated IgG; human; anti-SERPINE1 antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: Prior to conjugation, this antibody was specific for PAI-1 as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Form/Format: IgG-HRP conjugate as a clear, slightly red-brown liquid.
• Concentration: IgG-HRP concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)

• Application Notes: Suitable as a source of peroxidase labeled antibodies to PAI-1.
• Immunogen: Recombinant Plasminogen Activator Inhibitor-1 (PAI-1) prepared from bacterial extracts.
• Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Related Product Information for anti-SERPINE1 antibody

Type I plasminogen activator inhibitor, PAI-1, is a 50 kDa singlechain glycoprotein which belongs to the serine protease inhibitor (SERPIN) family of proteins. The concentration of PAI-1 in normal human plasma is very low, with an average concentration of approximately 20 ng/ml. The plasma concentrations of PAI-1 can be affected by a number of factors including diurnal variations, age, sex, pregnancy, obesity and exercise status. PAI-1 is synthesized by various cell types including endothelial cells, hepatocytes, vascular smooth muscle cells, fibroblasts, mesothelial cells, granulosa cells and malignant cell lines. It is also found in the alpha-granules of platelets as well as plasma. PAI-1 exists in at least three different conformations, including an active form with a half-life of approximately 1 - 3 hours, a latent form and a proteolytically or oxidatively inactivated form. The plasma glycoprotein vitronectin has been shown to bind active PAI-1 and stabilize it in its active conformation, extending its functional half-life to greater than 24 hours. Little specific binding of the latent or inactive forms of PAI-1 to vitronectin occurs. PAI-1 is considered to be the primary regulator of plasminogen activation in vivo. It is the major physiologic inhibitor of both the single chain and two-chain forms of tPA, being able to inhibit the single-chain form at least 10000 times greater than other PAIs.1-3

References

1. Kruithof, E.K.O. Plasminogen activator inhibitor type 1: biochemical, biological and clinical aspects. Fibrinolysis, 2:59-70, 1988.
2. Edelberg, J.M, Sane, D.C., and Pizzo, S.V. Vascular regulation of plasminogen activator inhibitor-1 activity. Semin. Thromb. Hemost., 20(4):319-323, 1994.
3. Loskutoff, D.J., Sawdey, M., and Mimuro, J. Type 1 plasminogen activator inhibitor, Prog. Thromb. Haemost., 9:87-115, 1988.

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.2
• UniProt Accession #: P05121
• Molecular Weight: 44929 Da
• NCBI Official Full Name: plasminogen activator inhibitor 1 isoform 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; OTTHUMP00000211478; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• Protein Family: Plasminogen activator inhibitor
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

Subunit structure: Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Ref.14 Ref.16

Subcellular location: Secreted.

Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Post-translational modification: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

Involvement in disease: Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [

MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Ref.15Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

Sequence similarities: Belongs to the serpin family.