Western Blot (WB)
(Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate,Lane 2: human SW620 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.)
Rabbit anti-Human PAFAH/PLA2G7 Polyclonal Antibody | anti-PAFAH antibody
Anti-PAFAH/PLA2G7 Antibody
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Immunogen Affinity Purified
Synonyms
PAFAH/PLA2G7; Polyclonal Antibody; Anti-PAFAH/PLA2G7 Antibody; Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2); PAF 2-acylhydrolase; PLA2G7; PAFAH; Phospholipase A2 group VII ; anti-PAFAH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
441
Applicable Applications for anti-PAFAH antibody
Western Blot (WB), ELISA Capture (EIA)
Application Notes
WB: 0.1-0.5 mug/ml
ELISA (Cap):1-5 mug/ml
Tested Species: In-house tested species with positive results.
ELISA (Cap):1-5 mug/ml
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human PAFAH recombinant protein (Position: A176-N415).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate,Lane 2: human SW620 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.)
Western Blot (WB)
(Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate,Lane 2: human SW620 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.)
Related Product Information for anti-PAFAH antibody
Description: Rabbit IgG polyclonal antibody for PAFAH detection. Tested with WB, ELISA (Cap) in Human.
Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2), also known as platelet-activating factor acetylhydrolase (PAF-AH), is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. In the blood it travels mainly with low-density lipoprotein (LDL). Less than 20% is associated with high-density lipoprotein HDL. It is an enzyme produced by inflammatory cells and hydrolyzes oxidized phospholipids in LDL. Lp-PLA2 is platelet-activating factor (PAF) acetylhydrolase, a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.
Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2), also known as platelet-activating factor acetylhydrolase (PAF-AH), is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. In the blood it travels mainly with low-density lipoprotein (LDL). Less than 20% is associated with high-density lipoprotein HDL. It is an enzyme produced by inflammatory cells and hydrolyzes oxidized phospholipids in LDL. Lp-PLA2 is platelet-activating factor (PAF) acetylhydrolase, a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.
References
1. Claud, E. C., Li, D., Xiao, Y., Caplan, M. S., Jilling, T. Platelet-activating factor regulates chloride transport in colonic epithelial cell monolayers. Pediat. Res. 52: 155-162, 2002. 2. Jang, Y., Kim, O. Y., Koh, S. J., Chae, J. S., Ko, Y. G., Kim, J. Y., Cho, H., Jeong, T.-S., Lee, W. S., Ordovas, J. M., Lee, J. H. The val279phe variant of the lipoprotein-associated phospholipase A2 gene is associated with catalytic activities and cardiovascular disease in Korean men. J. Clin. Endocr. Metab. 91: 3521-3527, 2006. 3. Lp-PLA2 Studies Collaboration. Lipoprotein-associated phospholipase A2 and risk of coronary disease, stroke, and mortality: collaborative analysis of 32 prospective studies.Lancet 375: 1536-1544, 2010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,077 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase
NCBI Official Synonym Full Names
phospholipase A2 group VII
NCBI Official Symbol
PLA2G7
NCBI Official Synonym Symbols
PAFAD; PAFAH; LP-PLA2; LDL-PLA2
NCBI Protein Information
platelet-activating factor acetylhydrolase
UniProt Protein Name
Platelet-activating factor acetylhydrolase
UniProt Gene Name
PLA2G7
UniProt Synonym Gene Names
PAFAH; PAF acetylhydrolase; gVIIA-PLA2; LDL-PLA(2)
NCBI Description
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Research Articles on PAFAH
Similar Products
Product Notes
The PAFAH pla2g7 (Catalog #AAA1751405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAFAH/PLA2G7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAFAH/PLA2G7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA Capture (EIA). WB: 0.1-0.5 mug/ml ELISA (Cap):1-5 mug/ml Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the PAFAH pla2g7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAFAH/PLA2G7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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