Rabbit anti-Human PAEL Receptor Polyclonal Antibody | anti-PAELR antibody

PAEL Receptor, NT (Probable G-protein Coupled Receptor 37, Endothelin B Receptor-like Protein-1, ETBR-LP-1, Parkin-associated Endothelin Receptor-like Receptor, PAELR, GPR37) (PE)

Cat. Num. AAA6491734

• Gene Names: GPR37; PAELR; EDNRBL; hET(B)R-LP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PAEL Receptor; Polyclonal Antibody; NT (Probable G-protein Coupled Receptor 37; Endothelin B Receptor-like Protein-1; ETBR-LP-1; Parkin-associated Endothelin Receptor-like Receptor; PAELR; GPR37) (PE); anti-PAELR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Pael-R.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PAELR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide in the N-terminal region of human Pael-R (GPR37) (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Pael-R (GPR37) Pab in mouse brain tissue lysate. Pael-R (GPR37) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Pael Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Pael antibody detected the Pael protein (arrow).)

Product Categories/Family for anti-PAELR antibody

Antibodies; G Protein Receptors

References

1. Yang, Y., et al. Neuron 37(6), 911-924 (2003). 2. Imai, Y., et al. Mol. Cell 10(1), 55-67 (2002). 3. Imai, Y., et al. Cell 105(7), 891-902 (2001). 4. Marazziti, D., et al. Genomics 45(1), 68-77 (1997). 5. Zeng, Z., et al. BBRC 233(2), 559-567 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4885323
• NCBI GeneID: 2861
• NCBI Accession #: NP_005293.1
• NCBI GenBank Nucleotide #: NM_005302.3
• UniProt Accession #: O15354; O00348; O14768; Q8TD39
• Molecular Weight: 67,114 Da
• NCBI Official Full Name: probable G-protein coupled receptor 37
• NCBI Official Synonym Full Names: G protein-coupled receptor 37 (endothelin receptor type B-like)
• NCBI Official Symbol: GPR37
• NCBI Official Synonym Symbols: PAELR; EDNRBL; hET(B)R-LP
• NCBI Protein Information: probable G-protein coupled receptor 37; ETBR-LP-1; endothelin B receptor-like protein 1; Parkin-associated endothelin receptor-like receptor
• UniProt Protein Name: Probable G-protein coupled receptor 37
• UniProt Gene Name: GPR37
• UniProt Synonym Gene Names: ETBR-LP-1; PAELR
• UniProt Entry Name: GPR37_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; ubiquitin ligase complex; receptor complex

Molecular Function: G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; heat shock protein binding; peptide receptor activity, G-protein coupled; Hsp70 protein binding; peptide binding

Biological Process: positive regulation of dopamine metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; G-protein signaling, adenylate cyclase inhibiting pathway; dopamine biosynthetic process; locomotion during locomotory behavior