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Western Blot (WB) (PABPN1L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PABPN1L antibody detected the PABPN1L protein (arrow).)

Rabbit PABPN1L Polyclonal Antibody | anti-PABPN1L antibody

PABPN1L Antibody (Center)

Gene Names
PABPN1L; ePABP2; PABPNL1
Reactivity
Human (Predicted Reactivity: Xenopus, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PABPN1L; Polyclonal Antibody; PABPN1L Antibody (Center); Embryonic polyadenylate-binding protein 2; Embryonic poly(A)-binding protein 2; ePABP-2; ePABP2; Embryonic poly(A)-binding protein type II; Poly(A)-binding protein nuclear-like 1; EPABP2; PABPNL1; anti-PABPN1L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Xenopus, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PABPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human PABPN1L.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Positions
166-194
Sequence Length
267
Applicable Applications for anti-PABPN1L antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PABPN1L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PABPN1L antibody detected the PABPN1L protein (arrow).)

Western Blot (WB) (PABPN1L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PABPN1L antibody detected the PABPN1L protein (arrow).)

Immunohistochemistry (IHC)

(PABPN1L Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PABPN1L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PABPN1L Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PABPN1L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PABPN1L Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of PABPN1L Antibody (Center) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(PABPN1L Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PABPN1L Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PABPN1L antibody
The function of this protein remains unknown.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30386
NCBI Official Full Name
embryonic polyadenylate-binding protein 2 isoform 1
NCBI Official Synonym Full Names
poly(A) binding protein, nuclear 1-like (cytoplasmic)
NCBI Official Symbol
PABPN1L
NCBI Official Synonym Symbols
ePABP2; PABPNL1
NCBI Protein Information
embryonic polyadenylate-binding protein 2
UniProt Protein Name
Embryonic polyadenylate-binding protein 2
UniProt Gene Name
PABPN1L
UniProt Synonym Gene Names
EPABP2; PABPNL1; Embryonic poly(A)-binding protein 2; ePABP-2; ePABP2
UniProt Entry Name
EPAB2_HUMAN

Uniprot Description

Pabpnl1: Binds the poly(A) tail of mRNA. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: cytoplasm

Molecular Function: RNA binding; nucleotide binding

Research Articles on PABPN1L

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Product Notes

The PABPN1L pabpn1l (Catalog #AAA9204210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 166-194. The PABPN1L Antibody (Center) reacts with Human (Predicted Reactivity: Xenopus, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PABPN1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PABPN1L pabpn1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PABPN1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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