Rabbit anti-Human PABPC3 Polyclonal Antibody | anti-PABPC3 antibody

Rabbit Polyclonal to Human PABPC3

Cat. Num. AAA248207

• Gene Names: PABPC3; PABP3; tPABP; PABPL3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PABPC3; Polyclonal Antibody; Rabbit Polyclonal to Human PABPC3; Anti-PABPC3 Antibody (aa107-323) IHC-plus; PABP3; TPABP; PABP-3; Poly(A)-binding protein 3; PABPL3; Poly(A)-binding protein-like 3; Testis PABP; Human PABPC3; anti-PABPC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human PABPC3
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
• Concentration: 0.83 mg/ml (varies by lot)
• Sequence Length: 631

• Applicable Applications for anti-PABPC3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000); Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Target Species: Human
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 107 and 323 of PABPC3 (SwissProt Q9H361). Percent identity by BLAST analysis: Human (100%); Rat (84%).
• Immunogen Type: Recombinant protein
• Immunogen: PABPC3 antibody was raised against recombinant fragment corresponding to a region within amino acids 107 and 323 of PABPC3 (SwissProt Q9H361). Percent identity by BLAST analysis: Human (100%); Rat (84%).
• Antigen Modification: aa107-323
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PABPC3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(IHC of paraffin-embedded SCM1 xenograft using PABPC3 antibody at 1:100 dilution.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: JurKat. 7.5% SDS PAGE. PABPC3 antibody diluted at 1:1000.)

NCBI and Uniprot Product Information

• NCBI GI #: 45238849
• NCBI GeneID: 5042
• NCBI Accession #: NP_112241.2
• NCBI GenBank Nucleotide #: NM_030979.2
• UniProt Accession #: Q9H361; Q8NHV0; Q9H086
• Molecular Weight: 70,031 Da
• NCBI Official Full Name: polyadenylate-binding protein 3
• NCBI Official Synonym Full Names: poly(A) binding protein, cytoplasmic 3
• NCBI Official Symbol: PABPC3
• NCBI Official Synonym Symbols: PABP3; tPABP; PABPL3
• NCBI Protein Information: polyadenylate-binding protein 3; PABP-3; testis PABP; poly(A)-binding protein 3; poly(A)-binding protein-like 3; testis-specific poly(A)-binding protein 3; testis-specific poly(A)-binding protein (PABP)
• UniProt Protein Name: Polyadenylate-binding protein 3
• UniProt Gene Name: PABPC3
• UniProt Synonym Gene Names: PABP3; PABPL3; PABP-3; Poly(A)-binding protein 3
• UniProt Entry Name: PABP3_HUMAN

NCBI Description

Messenger RNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). See PABPC1 (MIM 604679) for background information.[supplied by OMIM, Jul 2002]

Uniprot Description

PABP 3: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1. Belongs to the polyadenylate-binding protein type-1 family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 13q12-q13

Cellular Component: cytoplasm

Molecular Function: nucleotide binding; poly(A) binding

Biological Process: mRNA metabolic process