Rabbit anti-Human p95/NBS1 Polyclonal Antibody | anti-p95/NBS1 antibody

p95/NBS1 (Phospho-Ser343) Antibody

Cat. Num. AAA9413224

• Gene Names: NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: p95/NBS1; Polyclonal Antibody; p95/NBS1 (Phospho-Ser343) Antibody; NBN; anti-p95/NBS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 672

• Applicable Applications for anti-p95/NBS1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 343 (S-L-S(p)-Q-G) derived from Human p95/NBS1.
• Modification: Phospho-Ser343
• Target Name: p95/NBS1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1(Phospho-Ser343) Antibody.)

Related Product Information for anti-p95/NBS1 antibody

Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Product Categories/Family for anti-p95/NBS1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 67189945
• NCBI GeneID: 4683
• NCBI Accession #: NP_001019859.1
• NCBI GenBank Nucleotide #: NM_001024688.2
• UniProt Accession #: O60934; O60672; Q32NF7; Q53FM6; Q63HR6; Q7LDM2; B2R626; B2RNC5
• Molecular Weight: 84,959 Da
• NCBI Official Full Name: nibrin isoform 2
• NCBI Official Synonym Full Names: nibrin
• NCBI Official Symbol: NBN
• NCBI Official Synonym Symbols: ATV; NBS; P95; NBS1; AT-V1; AT-V2
• NCBI Protein Information: nibrin
• UniProt Protein Name: Nibrin
• UniProt Gene Name: NBN
• UniProt Synonym Gene Names: NBS; NBS1; P95
• UniProt Entry Name: NBN_HUMAN

NCBI Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Uniprot Description

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; isotype switching; positive regulation of kinase activity; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia