Rabbit p90 RSK Polyclonal Antibody | anti-RPS6KA1 antibody

p90 RSK Antibody

Cat. Num. AAA9503629

• Gene Names: RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: p90 RSK; Polyclonal Antibody; p90 RSK Antibody; 90 kDa ribosomal protein S6 kinase 1; kinase p90RSK1; KS6A1; KS6AA; MAPKAP-K1a; p90-RSK 1; pp90RSK1; Ribosomal protein S6 kinase alpha 1; Ribosomal S6 kinase 1; RPS6KA1; RSK-1; RSK1; S6K-alpha 1; anti-RPS6KA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: p90 RSK Antibody detects endogenous levels of total p90 RSK protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 719

• Applicable Applications for anti-RPS6KA1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human p90 RSK.
• Immunogen Range: 346-395
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p90 RSK Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from 293 cells, treated with PMA 125ng/ml 30', using p90 RSK Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 55743134
• NCBI GeneID: 6195
• NCBI Accession #: NP_001006666.1
• NCBI GenBank Nucleotide #: NM_001006665.1
• UniProt Accession #: Q15418; Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0; E9PRI4
• Molecular Weight: 82 kDa
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A1
• NCBI Official Symbol: RPS6KA1
• NCBI Official Synonym Symbols: RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA1
• UniProt Synonym Gene Names: MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).