Rabbit p70 S6K Polyclonal Antibody | anti-S6K antibody

p70 S6K (Phospho-S411) Polyclonal Antibody

Cat. Num. AAA3005292

• Gene Names: RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: p70 S6K; Polyclonal Antibody; p70 S6K (Phospho-S411) Polyclonal Antibody; Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70-S6K; P70S6K; Name: RPS6KB1; STK14A; p80 S6K1; p85 S6 kinase; p85 S6K; p85S6K; anti-S6K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-p70 S6K (S411) polyclonal antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at Ser411. This antibody also detects p85 S6 kinase when phosphorylated at Ser434.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 502

• Applicable Applications for anti-S6K antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human p70 S6K around the phosphorylation site of Serine 411.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-p70 S6K (S411) pAb in paraffin-embedded human breast carcinoma tissue.)

Related Product Information for anti-S6K antibody

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal.Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region. Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression. Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase.

NCBI and Uniprot Product Information

• NCBI GI #: 440546416
• NCBI GeneID: 6198
• NCBI Accession #: NP_001258989.1
• NCBI GenBank Nucleotide #: NP_001258989.1
• UniProt Accession #: P23443
• Molecular Weight: ~ 59, 70, 85kDa
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1 isoform e
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase B1
• NCBI Official Symbol: RPS6KB1
• NCBI Official Synonym Symbols: S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• NCBI Protein Information: ribosomal protein S6 kinase beta-1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• UniProt Gene Name: RPS6KB1
• UniProt Synonym Gene Names: STK14A; S6K-beta-1; S6K1; P70S6K1; p70-S6K 1; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
• UniProt Entry Name: KS6B1_HUMAN

NCBI Description

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; Translation; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1