Western Blot (WB)
(WB: Serum-starved (lane 1) and serum-treated (lane 2) 3T3 cell lysate was probed with MBS616425 at 0.2ug/ml.)
Rabbit anti-Human, Mouse p70 S6 Kinase Polyclonal Antibody
p70 S6 Kinase, phosphorylated, Thr412 (p70 S6K) (HRP)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
p70 S6 Kinase; Polyclonal Antibody; phosphorylated; Thr412 (p70 S6K) (HRP); anti-p70 S6 Kinase antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human p70 S6 kinase phosphorylated at Th 412 and the human splice variant of p70 S6 kinase phosphorylated at Thr 389. Species Crossreactivity: mouse. Species sequence homology: rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-p70 S6 Kinase antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 0.2-0.5ug/ml. Detects phospho-p70 S6 kinase in RIPA lysates from serum starved 3T3/A31 cells stimulated for 10 minutes with serum.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide aa405-416 (C-NQVFLGF[pT]YVAP-KK) corresponding to human p70 S6 kinase (KLH).
Conjugate
HRP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB: Serum-starved (lane 1) and serum-treated (lane 2) 3T3 cell lysate was probed with MBS616425 at 0.2ug/ml.)
Western Blot (WB)
(WB: Serum-starved (lane 1) and serum-treated (lane 2) 3T3 cell lysate was probed with MBS616425 at 0.2ug/ml.)
Product Categories/Family for anti-p70 S6 Kinase antibody
References
1. Avruch, J., Mol. Cell Biochem. 182: 31, 1998 2. Dufner, A. and G. Thomas, Exp. Cell Res. 25: 100, 1999.
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Product Notes
The p70 S6 Kinase (Catalog #AAA6491563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p70 S6 Kinase, phosphorylated, Thr412 (p70 S6K) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p70 S6 Kinase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 0.2-0.5ug/ml. Detects phospho-p70 S6 kinase in RIPA lysates from serum starved 3T3/A31 cells stimulated for 10 minutes with serum. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the p70 S6 Kinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p70 S6 Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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