Western Blot (WB)
(Western blot analysis of lysates from K562 cells treated with Starvation 24h, using p62 Dok (Phospho-Tyr398) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit p62 Dok Polyclonal Antibody | anti-DOK1 antibody
p62 Dok (Phospho-Tyr398) Antibody
Gene Names
DOK1; pp62; P62DOK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
p62 Dok; Polyclonal Antibody; p62 Dok (Phospho-Tyr398) Antibody; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62; DOK1; anti-DOK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
p62 Dok (Phospho-Tyr398) Antibody detects endogenous levels of p62 Dok only when phosphorylated at Tyr398.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
342
Applicable Applications for anti-DOK1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
ELISA: 1:20000
Target Modification
Phospho
Modification Sites
H:Y398; M:Y397; R:Y397
Immunogen
The antiserum was produced against synthesized peptide derived from human p62 Dok around the phosphorylation site of Tyr398.
Immunogen Range
365-414
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from K562 cells treated with Starvation 24h, using p62 Dok (Phospho-Tyr398) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from K562 cells treated with Starvation 24h, using p62 Dok (Phospho-Tyr398) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52 kDa
NCBI Official Full Name
docking protein 1 isoform b
NCBI Official Synonym Full Names
docking protein 1
NCBI Official Symbol
DOK1
NCBI Official Synonym Symbols
pp62; P62DOK
NCBI Protein Information
docking protein 1
UniProt Protein Name
Docking protein 1
Protein Family
UniProt Gene Name
DOK1
NCBI Description
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Uniprot Description
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Research Articles on DOK1
Similar Products
Product Notes
The DOK1 dok1 (Catalog #AAA9503002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p62 Dok (Phospho-Tyr398) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p62 Dok can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the DOK1 dok1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p62 Dok, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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