Guinea pig anti-Human p62 Polyclonal Antibody | anti-KHDRBS1 antibody

p62, NT

Cat. Num. AAA648507

• Gene Names: KHDRBS1; p62; p68; Sam68
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: p62; Polyclonal Antibody; NT; Anti -p62; anti-KHDRBS1 antibody

• Host: Guinea pig
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes p62 protein. The antiserum stains neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease. Species Crossreactivity: Human.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-KHDRBS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry (frozen and paraffin sections, enhanced after microwave treatment) and Immunoblot.; Dilution: Immunohistochemistry: 1:100; Incubation Time 1 hr at RT; extended with paraffin sections.; Western Blot: 1:1000 (ECL)
• Immunogen: N-terminal domain (20 amino acids) of human p62 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-KHDRBS1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 189500
• NCBI GeneID: 10657
• UniProt Accession #: Q07666; Q6PJX7; Q8NB97; Q99760; D3DPP3
• Molecular Weight: 48,227 Da
• NCBI Official Full Name: p62
• NCBI Official Synonym Full Names: KH domain containing, RNA binding, signal transduction associated 1
• NCBI Official Symbol: KHDRBS1
• NCBI Official Synonym Symbols: p62; p68; Sam68
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 1; src-associated in mitosis 68 kDa protein; p21 Ras GTPase-activating protein-associated p62; GAP-associated tyrosine phosphoprotein p62 (Sam68)
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• Protein Family: KH domain-containing, RNA-binding, signal transduction-associated protein
• UniProt Gene Name: KHDRBS1
• UniProt Synonym Gene Names: SAM68; Sam68
• UniProt Entry Name: KHDR1_HUMAN

NCBI Description

This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

Function: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (Ref.28). Ref.2 Ref.8 Ref.11 Ref.26 Ref.28Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase. Ref.2 Ref.8 Ref.11 Ref.26 Ref.28

Subunit structure: Self-associates to form homooligomers when bound to RNA, oligomerization appears to be limited when binding to proteins. Interacts with CBL, KHDRBS3, LCK, GRB2, JAK3, PIK3R1, PLCG1, PTPN6, RASA1, RBMY1A1 and STAT3. Interacts with PRMT1. Binds the WW domains of WBP4/FBP21, FNBP4/FBP30 and the SH3 domain of FYN through the Arg/Gly-rich-flanked Pro-rich regions

By similarity. Forms a complex with ILF2, ILF3, YLPM1, RBMX, NCOA5 and PPP1CA. Interacts with PTK6 (via SH3 and SH2 domains). Does not interact with TPR. Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.14 Ref.17 Ref.28

Subcellular location: Nucleus. Membrane Ref.1 Ref.10 Ref.12 Ref.15.

Tissue specificity: Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver. Ref.2

Developmental stage: Isoform 3 is only expressed in growth-arrested cells. Ref.2

Domain: The KH domain is required for binding to RNA

By similarity.The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions

By similarity.

Post-translational modification: Tyrosine phosphorylated by several non-receptor tyrosine kinases, LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins. Phosphorylation by PTK6 negatively regulates its RNA binding ability. Phosphorylation by PTK6 at Tyr-440 dictates the nulear localization of KHDRBS1. Ref.8 Ref.11 Ref.15Acetylated. Positively correlates with ability to bind RNA.Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine. Ref.1 Ref.7 Ref.12

Sequence similarities: Belongs to the KHDRBS family.Contains 1 KH domain.

Sequence caution: The sequence AAH10132.1 differs from that shown. Reason: Intron retention.