Testing Data
(Fig: Western blot analysis on A431 using anti-p53 polyclonal antibody)
anti-Mouse, Human p53 Polyclonal Antibody | anti-p53 antibody
p53 Antibody
Reactivity
Mouse, Human
Applications
Western Blot
Purity
Affinity purified
Synonyms
p53; Polyclonal Antibody; p53 Antibody; anti-p53 antibody
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of p53.
Purity/Purification
Affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
Sequence Length
391
Applicable Applications for anti-p53 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1,000
Preservative
0.05% Sodium Azide.
Cellular Localization
Cytoplasm; Nudeus
Positive Control
A431
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
(Fig: Western blot analysis on A431 using anti-p53 polyclonal antibody)
Testing Data
(Fig: Western blot analysis on A431 using anti-p53 polyclonal antibody)
Testing Data #2
Testing Data #2
Related Product Information for anti-p53 antibody
p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. It acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. P53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process.
Product Categories/Family for anti-p53 antibody
References
1. Levine, A.J. p53. the cellular gatekeeper for growth and division. Cell 88: 3.-3.1.(1.97). 2. Lakin, N.D. and S.P. Jackson. Regulation of p53.in response to DNA damage. Oncogene 1.: 7644-7655 (1.99). 3. Hoolstein, M., et al. p53.mutations in human cancers. Science 2.3. 49-53.1.91. 4. Harris C.C. p53. at the crossroads of molecular carcinogenesis and risk assessment. Science 2.2. 1.80-1.81.1.93.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~70kDa
NCBI Official Full Name
Transformation related protein 53
UniProt Protein Name
Cellular tumor antigen p53
Protein Family
UniProt Gene Name
Trp53
Uniprot Description
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.
Similar Products
Product Notes
The p53 trp53 (Catalog #AAA857592) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The p53 Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1,000. Researchers should empirically determine the suitability of the p53 trp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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