Western Blot (WB)
(Western blot analysis of P450R antibody (Center) in K562 cell line lysates (35ug/lane). P450R (arrow) was detected using the purified Pab.)
Rabbit anti-Human P450R Polyclonal Antibody | anti-POR antibody
P450R Antibody (Center)
Western Blot (WB)
(Western blot analysis of P450R antibody (Center) in K562 cell line lysates (35ug/lane). P450R (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of P450R antibody (Center) in K562 cell line lysates (35ug/lane). P450R (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(P450R Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P450R Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(P450R Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P450R Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Mast,N., Arch. Biochem. Biophys. 483 (1), 81-89 (2009)
Brenner,S., FEBS J. 275 (18), 4540-4557 (2008)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Protein type: EC 1.6.2.4; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q11.2
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; mitochondrion
Molecular Function: protein binding; enzyme binding; FAD binding; electron carrier activity; FMN binding; NADPH-hemoprotein reductase activity; hydrolase activity; cytochrome-b5 reductase activity; nitric oxide dioxygenase activity; iron ion binding; NADP binding; iron-cytochrome-c reductase activity
Biological Process: response to drug; nitric oxide catabolic process; internal peptidyl-lysine acetylation; positive regulation of monooxygenase activity; positive regulation of cholesterol biosynthetic process; negative regulation of caspase activity; negative regulation of lipase activity; nitrate catabolic process; positive regulation of chondrocyte differentiation; flavonoid metabolic process; positive regulation of smoothened signaling pathway; carnitine metabolic process; fatty acid oxidation; response to nutrient
Disease: Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency; Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis
Research Articles on POR
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Product Notes
The POR por (Catalog #AAA9207892) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 462-488. The P450R Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P450R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the POR por for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P450R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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