Rabbit anti-Human p40 Polyclonal Antibody | anti-p40 antibody

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)

Cat. Num. AAA4381362

• Gene Names: TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: p40; Polyclonal Antibody; p40 (deltaNp63) (Squamous; Basal & Myoepithelial Cell Marker); deltaNp63; TP63; anti-p40 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody s ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 555

• Applicable Applications for anti-p40 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human prostate or lung tissue (IHC).
• Immunogen: Recombinant human p40 protein fragment
• Cellular Localization: Nuclear
• Hu-chromosome Location: 3q28
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with p40 Rabbit Polyclonal Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Cervix stained with p40 Rabbit Polyclonal Antibody.)

References

1. Terry J, et al. Am J Surg Pathol. 2010; 34(12):1805-11. 2. Signoretti S, et al. Am J Pathol. 2000; 157(6):1769-75.

NCBI and Uniprot Product Information

• NCBI GI #: 169234657
• NCBI GeneID: 8626
• NCBI Accession #: NP_001108450.1
• NCBI GenBank Nucleotide #: NP_001108450.1
• UniProt Accession #: Q9H3D4
• Molecular Weight: 40kDa
• NCBI Official Full Name: tumor protein 63 isoform 2
• NCBI Official Synonym Full Names: tumor protein p63
• NCBI Official Symbol: TP63
• NCBI Official Synonym Symbols: AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
• NCBI Protein Information: tumor protein 63
• UniProt Protein Name: Tumor protein 63
• Protein Family: Structural glycoprotein
• UniProt Gene Name: TP63
• UniProt Synonym Gene Names: KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L
• UniProt Entry Name: P63_HUMAN

NCBI Description

This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016]

Uniprot Description

p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: nucleoplasm; transcription factor complex; rough endoplasmic reticulum; dendrite; cytoplasm; nuclear chromatin; cytosol; nucleus; chromatin

Molecular Function: identical protein binding; protein binding; DNA binding; p53 binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; WW domain binding; damaged DNA binding; chromatin binding; transcription factor activity

Biological Process: G1 DNA damage checkpoint; ectoderm and mesoderm interaction; apoptosis; positive regulation of transcription, DNA-dependent; cloacal septation; epidermal cell division; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; protein homotetramerization; smooth muscle development; polarized epithelial cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; sympathetic nervous system development; regulation of neuron apoptosis; positive regulation of mesenchymal cell proliferation; epithelial cell development; response to gamma radiation; establishment of planar polarity; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; skeletal development; female genitalia morphogenesis; proximal/distal pattern formation; positive regulation of Notch signaling pathway; embryonic limb morphogenesis; response to X-ray; regulation of epidermal cell division; Notch signaling pathway; hair follicle morphogenesis; transcription, DNA-dependent; urinary bladder development; negative regulation of keratinocyte differentiation; multicellular organismal aging; keratinocyte proliferation; replicative cell aging; odontogenesis of dentine-containing teeth; keratinocyte differentiation; chromatin remodeling; positive regulation of osteoblast differentiation; neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Ankyloblepharon-ectodermal Defects-cleft Lip/palate; Rapp-hodgkin Syndrome; Adult Syndrome; Ectrodactyly, Ectodermal Dysplasia, And Cleft Lip/palate Syndrome 3; Split-hand/foot Malformation 4; Limb-mammary Syndrome