Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using p40 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit p40 Polyclonal Antibody | anti-RABEPK antibody
p40 Antibody
Gene Names
RABEPK; p40; RAB9P40; bA65N13.1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
p40; Polyclonal Antibody; p40 Antibody; 40 kDa Rab9 effector protein; 8430412M01Rik; 9530020d24rik; AV073337; bA65N13.1; C87311; DKFZp686P1077; OTTHUMP00000022126; OTTMUSP00000012898; OTTMUSP00000012899; OTTMUSP00000041318; RAB9 effector p40; Rab9 effector protein with Kelch motifs; RAB9p40; RABEK_HUMAN; RABEPK; RABEPK protein; RGD1310612; RP11 65N13.1; RP23-446N16.2; anti-RABEPK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
p40 Antibody detects endogenous levels of total p40.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
372
Applicable Applications for anti-RABEPK antibody
ELISA (EIA)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ICC: 1:100-1:500
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human p40, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using p40 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using p40 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612463 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612463 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612463 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612463 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Related Product Information for anti-RABEPK antibody
Function: Rab9 effector required for endosome to trans-Golgi network (TGN) transport.
Post Translational Modifications: Phosphorylated on Ser residues most probably by PIP5K3.
Subcellular Location: Cytoplasm. Endosome membrane. Note: Interaction with PIP5K3 and subsequent phosphorylation recruits it to the endosomal membrane.
Subunit Structure: Interacts with PIP5K3. Interacts with RAB9 in its GTP-bound conformation.
Post Translational Modifications: Phosphorylated on Ser residues most probably by PIP5K3.
Subcellular Location: Cytoplasm. Endosome membrane. Note: Interaction with PIP5K3 and subsequent phosphorylation recruits it to the endosomal membrane.
Subunit Structure: Interacts with PIP5K3. Interacts with RAB9 in its GTP-bound conformation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)40kD.
Predicted Molecular Weight: (Calculated)41kDa.
Predicted Molecular Weight: (Calculated)41kDa.
NCBI Official Full Name
Rab9 effector protein with kelch motifs
NCBI Official Synonym Full Names
Rab9 effector protein with kelch motifs
NCBI Official Symbol
RABEPK
NCBI Official Synonym Symbols
p40; RAB9P40; bA65N13.1
NCBI Protein Information
rab9 effector protein with kelch motifs
UniProt Protein Name
Rab9 effector protein with kelch motifs
Protein Family
UniProt Gene Name
RABEPK
UniProt Synonym Gene Names
RAB9P40
UniProt Entry Name
RABEK_HUMAN
Uniprot Description
RABEPK: Rab9 effector required for endosome to trans-Golgi network (TGN) transport. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: intracellular membrane-bound organelle; plasma membrane; endosome membrane; endosome
Biological Process: receptor-mediated endocytosis; vesicle docking during exocytosis
Research Articles on RABEPK
Similar Products
Product Notes
The RABEPK rabepk (Catalog #AAA9612463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p40 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's p40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the RABEPK rabepk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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