Rabbit p38 MAPK Polyclonal Antibody | anti-p38 antibody

RABBIT ANTI p38 MAPK (pThr180/Tyr182)

Cat. Num. AAA221795

• Gene Names: Mapk14; RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human, Monkey (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Western Blot
• Synonyms: p38 MAPK; Polyclonal Antibody; RABBIT ANTI p38 MAPK (pThr180/Tyr182); anti-p38 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human, Monkey (Expected from Sequence), Mouse (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for p38 mitogen-activated protein kinase (p38 MAPK), also known as MAPK14, when phosphorylated at threonine 180 and tyrosine 182. ; ; p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. ; ; The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 360

• Applicable Applications for anti-p38 antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item recognises a band of approximately 39kD in Western blots of anisomycin C-6 glioma cell lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182.; Buffer Solution
• Antiserum Preparation: Antisera to phosphorylated rat p38 MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-p38 antibody

Rabbit anti p38MAPK (pThr180/tyr182 antibody recognizes p38 mitogen-activated protein kinase (p38 MAPK), also known as MAPK14, when phosphorylated at threonine 180 and tyrosine 182. p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. p38MAPK is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.

NCBI and Uniprot Product Information

• NCBI GI #: 2499601
• NCBI GeneID: 81649
• UniProt Accession #: P70618; O08594; Q99MG4
• Molecular Weight: 41,521 Da
• NCBI Official Full Name: Mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen activated protein kinase 14
• NCBI Official Symbol: Mapk14
• NCBI Official Synonym Symbols: RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• NCBI Protein Information: mitogen-activated protein kinase 14; Csaids binding protein; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAPK 14; MAX-interacting protein 2; cytokine suppressive anti-inflammatory drug binding protein; cytokine-supressive anti-inflammatory drug binding protein; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; stress-activated protein kinase 2A
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: Mapk14
• UniProt Synonym Gene Names: Csbp1; Csbp2; MAP kinase 14; MAPK 14; MAP kinase p38 alpha
• UniProt Entry Name: MK14_RAT

NCBI Description

mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]

Uniprot Description

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cell; cytoplasm; nucleus; cytosol

Molecular Function: protein C-terminus binding; MAP kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; protein amino acid autophosphorylation; stress-activated MAPK cascade; cell morphogenesis; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of glucose import; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; response to glucose stimulus; lipopolysaccharide-mediated signaling pathway; chondrocyte differentiation; angiogenesis; placenta development; cartilage condensation; positive regulation of cardiac muscle cell proliferation; skeletal muscle development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; positive regulation of blood vessel endothelial cell migration; MAPKKK cascade; DNA damage response, signal transduction; glucose metabolic process; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus; myoblast cell differentiation involved in skeletal muscle regeneration