P2X2 Polyclonal Antibody | anti-P2X2 antibody

Anti-P2X2 Antibody

Cat. Num. AAA178008

• Gene Names: P2RX2; P2X2; DFNA41
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: P2X2; Polyclonal Antibody; Anti-P2X2 Antibody; P2X purinoceptor 2; ATP receptor; MGC129601; P2RX 2; P2RX2; P2RX2_HUMAN; P2X Receptor subunit 2; P2X2a; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 2; Purinergic receptor P2X2 isoform A; Purinergic receptor P2X2 isoform B; Purinergic receptor P2X2 isoform C; Purinergic receptor P2X2 isoform D; Purinergic receptor P2X2 isoform H; Purinergic receptor P2X2 isoform I; purinergic receptor P2X; ligand gated ion channel; 2; anti-P2X2 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 370

• Applicable Applications for anti-P2X2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- P2X2 Picoband antibody, MBS178008, Western blottingAll lanes: Anti P2X2 (MBS178008) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: SHG Whole Cell Lysate at 40ugLane 6: NEURO Whole Cell Lysate at 40ugLane 7: 22RV1 Whole Cell Lysate at 40ugLane 8: U87 Whole Cell Lysate at 40ugPredicted bind size: 52KDObserved bind size: 60KD )

Immunohistochemistry (IHC)

(Anti- P2X2 Picoband antibody, MBS178008, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-P2X2 antibody

Description: Rabbit IgG polyclonal antibody for P2X purinoceptor 2(P2RX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: The P2RX2 gene encodes the P2X2 receptor, which assembles as a trimer to form a ligand-gated ion channel gated by extracellular ATP. P2X2 receptors mediate a variety of cellular responses, including excitatory postsynaptic responses in sensory neurons. The product of this gene belongs to the family of purinoceptors for ATP. P2RX2 is mapped to 12q24.33. It has been found that ATP-activated P2RX2 influenced OHC electromotility, a stimulus-induced change in hair cell length that functions as an amplifier to determine hearing sensitivity and frequency selectivity. What's more, P2RX2 channels were necessary for development of the temporary threshold shift.

References

1. Housley, G. D., Morton-Jones, R., Vlajkovic, S. M., Telang, R. S., Paramananthasivam, V., Tadros, S. F., Wong, A. C. Y., Froud, K. E., Cederholm, J. M. E., Sivakumaran, Y., Snguanwongchai, P., Khakh, B. S., Cockayne, D. A., Thorne, P. R., Ryan, A. F. ATP-gated ion channels mediate adaptation to elevated sound levels. Proc. Nat. Acad. Sci. 110: 7494-7499, 2013. 2. Yan, D., Zhu, Y., Walsh, T., Xie, D., Yuan, H., Sirmaci, A., Fujikawa, T., Wong, A. C. Y., Loh, T. L., Du, L., Grati, M., Vlajkovic, S. M., and 10 others. Mutation of the ATP-gated P2X(2) receptor leads to progressive hearing loss and increased susceptibility to noise. Proc. Nat. Acad. Sci. 110: 2228-2233, 2013. 3. Yu, N., Zhao, H.-B. ATP activates P2x receptors and requires extracellular Ca(++) participation to modify outer hair cell nonlinear capacitance. Pflugers Arch. 457: 453-461, 2008.

NCBI and Uniprot Product Information

• NCBI GI #: 531990826
• NCBI GeneID: 22953
• NCBI Accession #: NP_001269093.1
• NCBI GenBank Nucleotide #: NM_001282164.1
• UniProt Accession #: Q9UBL9; Q6V9R6; Q9NR37; Q9NR38; Q9UHD5; Q9UHD6; Q9UHD7; A6NGB4; A6NH93; A6NHC2; A6NHU3; A6NIG9
• Molecular Weight: 41,370 Da
• NCBI Official Full Name: P2X purinoceptor 2 isoform J
• NCBI Official Synonym Full Names: purinergic receptor P2X 2
• NCBI Official Symbol: P2RX2
• NCBI Official Synonym Symbols: P2X2; DFNA41
• NCBI Protein Information: P2X purinoceptor 2
• UniProt Protein Name: P2X purinoceptor 2
• UniProt Gene Name: P2RX2
• UniProt Synonym Gene Names: P2X2; P2X2
• UniProt Entry Name: P2RX2_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

P2X2: Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Belongs to the P2X receptor family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, cation; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: apical plasma membrane; integral to nuclear inner membrane; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: ATP binding; ATP-gated cation channel activity; identical protein binding; ligand-gated ion channel activity; purinergic nucleotide receptor activity

Biological Process: behavioral response to pain; blood coagulation; detection of hypoxic conditions in blood by carotid body chemoreceptors; neuromuscular junction development; neuromuscular synaptic transmission; peristalsis; positive regulation of calcium-mediated signaling; protein homooligomerization; response to ATP; response to carbohydrate stimulus; response to hypoxia; sensory perception of sound; sensory perception of taste; skeletal muscle fiber development; urinary bladder smooth muscle contraction

Disease: Deafness, Autosomal Dominant 41