Rabbit anti-Human P2R3C Polyclonal Antibody | anti-P2R3C antibody

P2R3C, NT (PPP2R3C, C14orf10, G5PR, Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma, Protein phosphatase subunit G5PR, Rhabdomyosarcoma antigen MU-RMS-40.6A/6C) (FITC)

Cat. Num. AAA6330178

• Gene Names: PPP2R3C; G4-1; G5pr; C14orf10
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: P2R3C; Polyclonal Antibody; NT (PPP2R3C; C14orf10; G5PR; Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma; Protein phosphatase subunit G5PR; Rhabdomyosarcoma antigen MU-RMS-40.6A/6C) (FITC); PPP2R3C; Rhabdomyosarcoma antigen MU-RMS-40.6A/6C; anti-P2R3C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-P2R3C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: P2R3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~87 amino acids from the N-terminal region of human P2R3C.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of P2R3C Antibody (N-term) in Y79 cell line lysates (35ug/lane). P2R3C (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(P2R3C Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P2R3C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-P2R3C antibody

P2R3C may regulate MCM3AP phosphorylation through phosphatase recruitment. It may play a role in the activation-induced cell death of B-cells (By similarity).

Product Categories/Family for anti-P2R3C antibody

Antibodies; Enzymes; Phosphatase

References

Xing, Y., et al. J. Exp. Med. 202(5):707-719(2005). Kamnasaran, D., et al. Genomics 85(5):608-621(2005). Kono, Y., et al. Genes Cells 7(8):821-834(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 31542242
• NCBI GeneID: 55012
• NCBI Accession #: NP_060387.2
• NCBI GenBank Nucleotide #: NM_017917.2
• UniProt Accession #: Q969Q6; Q5GJ55; Q5GJ56; Q6P4G2; Q86TZ3; Q9NWR9; B4DEN7; D3DS97; D3DS98
• Molecular Weight: 53,316 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit B'', gamma
• NCBI Official Symbol: PPP2R3C
• NCBI Official Synonym Symbols: G4-1; G5pr; C14orf10
• NCBI Protein Information: serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma; protein phosphatase subunit G5PR; rhabdomyosarcoma antigen MU-RMS-40.6A; rhabdomyosarcoma antigen Mu-RMS-40.6C; rhabdomyosarcoma antigen MU-RMS-40.6A/6C; protein phosphatase 2 (
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma
• UniProt Gene Name: PPP2R3C
• UniProt Synonym Gene Names: C14orf10; G5PR
• UniProt Entry Name: P2R3C_HUMAN

NCBI Description

This gene encodes a regulatory subunit of the serine/threonine phosphatase, protein phosphatase 2. This protein is localized to both nuclear and cytoplasmic regions depending on cell cycle phase. Homozygous conditional knockout mice for this gene exhibit reduced numbers and impaired proliferation of immune system B cells. This protein may regulate the expression of the P-glycoprotein ATP-binding cassette transporter through its phosphatase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

PPP2R3C: May regulate MCM3AP phosphorylation through phosphatase recruitment. May play a role in the activation-induced cell death of B-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 14q13.2

Cellular Component: centrosome; cytoplasm; spindle; nucleus

Molecular Function: metal ion binding

Biological Process: spleen development; B cell homeostasis; activation of protein kinase activity; regulation of antimicrobial humoral response; positive regulation of B cell differentiation; T cell homeostasis; cortical cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; regulation of mitochondrial depolarization