Rabbit anti-Human p21Cip1/WAF1 Polyclonal Antibody | anti-PPM1G antibody

p21Cip1/WAF1 (Cyclin-dependent Kinase Inhibitor 1, CDK-interacting Protein 1, Melanoma Differentiation-associated Protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1)

Cat. Num. AAA628234

• Gene Names: PPM1G; PP2CG; PPP2CG; PP2CGAMMA
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: p21Cip1/WAF1; Polyclonal Antibody; p21Cip1/WAF1 (Cyclin-dependent Kinase Inhibitor 1; CDK-interacting Protein 1; Melanoma Differentiation-associated Protein 6; MDA-6; p21; CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1); Anti -p21Cip1/WAF1 (Cyclin-dependent Kinase Inhibitor 1; anti-PPM1G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human p21Cip1/WAF1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-PPM1G antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic non-phosphopeptide corresponding to human p21Cip1 around the phosphorylation site of threonine 145 (R-Q-TP-S-M).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PPM1G antibody

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and overexpression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Product Categories/Family for anti-PPM1G antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 29826282
• NCBI GeneID: 5496
• NCBI Accession #: NP_817092.1
• NCBI GenBank Nucleotide #: NM_177983.2
• UniProt Accession #: O15355
• Molecular Weight: 59,272 Da
• NCBI Official Full Name: protein phosphatase 1G
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1G
• NCBI Official Symbol: PPM1G
• NCBI Official Synonym Symbols: PP2CG; PPP2CG; PP2CGAMMA
• NCBI Protein Information: protein phosphatase 1G; PP2C-gamma; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• UniProt Protein Name: Protein phosphatase 1G
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1G
• UniProt Synonym Gene Names: PPM1C; PP2C-gamma
• UniProt Entry Name: PPM1G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Uniprot Description

PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Protein type: Spliceosome; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: cell cycle arrest; protein amino acid dephosphorylation