Immunohistochemistry (IHC)
Rabbit anti-Human p16INK4a Polyclonal Antibody | anti-p16INK4a antibody
p16INK4a (CT)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification
Synonyms
p16INK4a; Polyclonal Antibody; p16INK4a (CT); ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A; anti-p16INK4a antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This antibody recognizes ~16-18 kDa of human p16INK4a protein. The other species are not tested
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
100ug/200ul (varies by lot)
Applicable Applications for anti-p16INK4a antibody
Western Blot (WB), Immunohistochemistry (IHC)-Paraffin
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation2-5 ug/ml
IHC: 2-10 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation2-5 ug/ml
IHC: 2-10 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the C-terminus of human p16INK4a protein.
Preparation and Storage
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Related Product Information for anti-p16INK4a antibody
The INK4 (inhibitor of cyclin-dependent kinase 4) family consists of four tumor-suppressor proteins: p15(INK4B), p16(INK4A), p18(INK4C), and p19(INK4D). While their sequences and structures are highly homologous, they show appreciable differences in conformational flexibility, stability, and aggregation tendency. p16 (INK4a, CDKN2A, cyclin dependent kinase inhibitor 2A) is a tumor suppressor gene that inhibits cdk4 and cdk6 which phosphorylate the Rb protein. P16INK4a overexpression has been demonstrated in cervical cancers as a result of functional inactivation of pRb. Thus, this antibody can be an important marker for dysplastic squamous and glandular cells of the cervix.
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Product Notes
The p16INK4a (Catalog #AAA462262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p16INK4a (CT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p16INK4a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC)-Paraffin. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation2-5 ug/ml IHC: 2-10 ug/ml . Researchers should empirically determine the suitability of the p16INK4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16INK4a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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