Western Blot (WB)
(Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates.)
Rabbit p130 Cas (pY249) Polyclonal Antibody | anti-BCAR1 antibody
Anti-p130 Cas (pY249) Phospho Antibody
Western Blot (WB)
(Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
Uniprot Description
P130Cas: a docking protein containing multiple protein-protein interaction domains. Plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. The amino-terminal SH3 domain regulates its interaction with focal adhesion kinase (FAK) and the FAK-related kinase PYK2 and also with tyrosine phosphatases PTP-1B and PTP-PEST. Overexpression confers antiestrogen resistance on breast cancer cells.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component: ruffle; focal adhesion; lamellipodium; cytoplasm; nucleolus; plasma membrane; cytosol; actin cytoskeleton
Molecular Function: signal transducer activity; protein binding; protein kinase binding; SH3 domain binding
Biological Process: antigen receptor-mediated signaling pathway; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; nerve growth factor receptor signaling pathway; actin filament organization; hepatocyte growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; T cell receptor signaling pathway; regulation of apoptosis; cell proliferation; G-protein coupled receptor protein signaling pathway; B cell receptor signaling pathway; cell division; insulin receptor signaling pathway; regulation of cell growth; cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cell migration
Research Articles on BCAR1
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Product Notes
The BCAR1 bcar1 (Catalog #AAA822260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p130 Cas (pY249) Phospho Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's p130 Cas (pY249) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC). WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500). Researchers should empirically determine the suitability of the BCAR1 bcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130 Cas (pY249), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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