IP (Immunoprecipitation)
(Immunoprecipitating FNTB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
FNTB recombinant antibody
FNTB Antibody
Gene Names
FNTB; FPTB
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
FNTB, Recombinant Antibody; FNTB Antibody; Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta); FNTB; FNTB recombinant antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
5H4
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for FNTB recombinant antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antibody Type
Recombinant Antibody
Conjugation
Non-conjugated
Immunogen
A synthesized peptide derived from human FNTB
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating FNTB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating FNTB in K562 whole cell lysateLane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: K562 whole cell lysate?500ug?Lane 3: K562 whole cell lysate (10ug))
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissueAll lanes: FNTB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49, 44 kDaObserved band size: 49 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissueAll lanes: FNTB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49, 44 kDaObserved band size: 49 kDa)
Related Product Information for FNTB recombinant antibody
Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
Product Categories/Family for FNTB recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,774 Da
NCBI Official Full Name
protein farnesyltransferase subunit beta
NCBI Official Synonym Full Names
farnesyltransferase, CAAX box, beta
NCBI Official Symbol
FNTB
NCBI Official Synonym Symbols
FPTB
NCBI Protein Information
protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; ras proteins prenyltransferase subunit beta
UniProt Protein Name
Protein farnesyltransferase subunit beta
UniProt Gene Name
FNTB
UniProt Synonym Gene Names
FTase-beta
UniProt Entry Name
FNTB_HUMAN
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Product Notes
The FNTB fntb (Catalog #AAA243882) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FNTB Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FNTB can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the FNTB fntb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FNTB, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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