Western Blot (WB)
(OTUD6B Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the OTUD6B antibody detected OTUD6B protein (arrow).)
Rabbit anti-Human, Mouse OTUD6B Polyclonal Antibody | anti-OTUD6B antibody
OTUD6B, CT (OTUD6B, DUBA5, OTU domain-containing protein 6B, DUBA-5)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
OTUD6B; Polyclonal Antibody; CT (OTUD6B; DUBA5; OTU domain-containing protein 6B; DUBA-5); Anti -OTUD6B; anti-OTUD6B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human OTUD6B. Species Crossreactivity: mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-OTUD6B antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry and ELISA.
Dilution: Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Dilution: Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunogen
Synthetic peptide corresponding to a region between aa241-270 from the C-terminal region of human OTUD6B, conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(OTUD6B Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the OTUD6B antibody detected OTUD6B protein (arrow).)
Western Blot (WB)
(OTUD6B Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the OTUD6B antibody detected OTUD6B protein (arrow).)
Flow Cytometry (FC/FACS)
(OTUD6B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(OTUD6B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-OTUD6B antibody
Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA5 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].
Product Categories/Family for anti-OTUD6B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,542 Da
NCBI Official Full Name
OTU domain-containing protein 6B
NCBI Official Synonym Full Names
OTU domain containing 6B
NCBI Official Symbol
OTUD6B
NCBI Protein Information
OTU domain-containing protein 6B
UniProt Protein Name
OTU domain-containing protein 6B
Protein Family
UniProt Gene Name
OTUD6B
UniProt Entry Name
OTU6B_CHICK
Uniprot Description
Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities: Contains 1 OTU domain.
Similar Products
Product Notes
The OTUD6B otud6b (Catalog #AAA645689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OTUD6B, CT (OTUD6B, DUBA5, OTU domain-containing protein 6B, DUBA-5) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OTUD6B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry and ELISA. Dilution: Western Blot: 1:100-1:500 Flow Cytometry: 1:10-1:50. Researchers should empirically determine the suitability of the OTUD6B otud6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUD6B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
