Rabbit anti-Human OTUB2 Polyclonal Antibody | anti-OTUB2 antibody

OTUB2, NT (OTUB2, C14orf137, OTB2, OTU2, Ubiquitin thioesterase OTUB2, Deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, Otubain-2, Ubiquitin-specific-processing protease OTUB2) (PE)

Cat. Num. AAA6329888

• Gene Names: OTUB2; OTB2; OTU2; C14orf137
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OTUB2; Polyclonal Antibody; NT (OTUB2; C14orf137; OTB2; OTU2; Ubiquitin thioesterase OTUB2; Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2) (PE); Ubiquitin-specific-processing protease OTUB2; anti-OTUB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-OTUB2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: OTUB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human OTUB2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(OTUB2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the OTUB2 antibody detected the OTUB2 protein (arrow).)

Related Product Information for anti-OTUB2 antibody

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Product Categories/Family for anti-OTUB2 antibody

Antibodies; Ubiquitin

References

Li, S., et al. J. Biol. Chem. 285(7):4291-4297(2010). Sowa, M.E., et al. Cell 138(2):389-403(2009). Edelmann, M.J., et al. Biochem. J. 418(2):379-390(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Nanao, M.H., et al. EMBO Rep. 5(8):783-788(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 12962939
• NCBI GeneID: 78990
• NCBI Accession #: NP_075601.1
• NCBI GenBank Nucleotide #: NM_023112.3
• UniProt Accession #: Q96DC9; Q6IA10; Q9H6T1
• Molecular Weight: 8,637 Da
• NCBI Official Full Name: ubiquitin thioesterase OTUB2
• NCBI Official Synonym Full Names: OTU deubiquitinase, ubiquitin aldehyde binding 2
• NCBI Official Symbol: OTUB2
• NCBI Official Synonym Symbols: OTB2; OTU2; C14orf137
• NCBI Protein Information: ubiquitin thioesterase OTUB2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; deubiquitinating enzyme OTUB2; otubain-2; ubiquitin-specific protease otubain 2; ubiquitin-specific-processing protease OTU
• UniProt Protein Name: Ubiquitin thioesterase OTUB2
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: OTUB2
• UniProt Synonym Gene Names: C14orf137; OTB2; OTU2
• UniProt Entry Name: OTUB2_HUMAN

NCBI Description

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]

Uniprot Description

OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Hydrolase; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: nucleus

Molecular Function: ubiquitin-specific protease activity

Biological Process: protein deubiquitination; proteolysis