Western Blot (WB)
(Western blot analysis of OSGEP expression in Raji (A), Jurkat (B), mouse heart (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Rabbit OSGEP Polyclonal Antibody | anti-OSGEP antibody
Anti-OSGEP Antibody
Gene Names
OSGEP; KAE1; GCPL1; OSGEP1; PRSMG1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
OSGEP; Polyclonal Antibody; Anti-OSGEP Antibody; GCPL1; Probable tRNA N6-adenosine threonylcarbamoyltransferase; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; O-sialoglycoprotein endopeptidase; hOSGEP; t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP; tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP; anti-OSGEP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of OSGEP protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
335
Applicable Applications for anti-OSGEP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human OSGEP
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of OSGEP expression in Raji (A), Jurkat (B), mouse heart (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of OSGEP expression in Raji (A), Jurkat (B), mouse heart (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of OSGEP staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of OSGEP staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-OSGEP antibody
Rabbit polyclonal antibody to OSGEP
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,427 Da
NCBI Official Full Name
probable tRNA N6-adenosine threonylcarbamoyltransferase
NCBI Official Synonym Full Names
O-sialoglycoprotein endopeptidase
NCBI Official Symbol
OSGEP
NCBI Official Synonym Symbols
KAE1; GCPL1; OSGEP1; PRSMG1
NCBI Protein Information
probable tRNA N6-adenosine threonylcarbamoyltransferase
UniProt Protein Name
Probable tRNA N6-adenosine threonylcarbamoyltransferase
Protein Family
UniProt Gene Name
OSGEP
UniProt Synonym Gene Names
t(6)A synthase; hOSGEP
UniProt Entry Name
OSGEP_HUMAN
Uniprot Description
OSGEP: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Belongs to the KAE1 / YgjD family.
Protein type: EC 2.6.99.4; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: cytoplasm; nucleus
Molecular Function: metal ion binding
Research Articles on OSGEP
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Product Notes
The OSGEP osgep (Catalog #AAA8245675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OSGEP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OSGEP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the OSGEP osgep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSGEP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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