Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS629248. OTC (arrow) was detected using the purified Pab.)
Rabbit anti-Human Ornithine Carbamoyltransferase Polyclonal Antibody | anti-OTC antibody
Ornithine Carbamoyltransferase (OTC, Ornithine Transcarbamylase, OTCase, MGC129967, MGC129968, MGC138856, OCTD)
Gene Names
OTC; F1B16.13; F1B16_13; ornithine carbamoyltransferase; ORNITHINE CARBAMOYLTRANSFERASE; OTC
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Ornithine Carbamoyltransferase; Polyclonal Antibody; Ornithine Carbamoyltransferase (OTC; Ornithine Transcarbamylase; OTCase; MGC129967; MGC129968; MGC138856; OCTD); Anti -Ornithine Carbamoyltransferase (OTC; anti-OTC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human OTC at ~40 kD
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Concentration
0.55 mg/ml (varies by lot)
Applicable Applications for anti-OTC antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry
Application Notes
WB: 1:100-1:500
IHC (FFPE): 1:10-1:50
FC: 1:10-1:50
IHC (FFPE): 1:10-1:50
FC: 1:10-1:50
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 71-98 in the central region of human OTC, UniProt Accession #NP_000522.3, P00480.
Species Sequence Homology: Mouse, Rat, Bovine and Porcine.
Species Sequence Homology: Mouse, Rat, Bovine and Porcine.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS629248. OTC (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS629248. OTC (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatoarcinoma using MBS629248, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629248 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatoarcinoma using MBS629248, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629248 for immunohistochemistry.)
Testing Data
(Flow Cytometric analysis of ATDC5 cells using MBS629248 (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Testing Data
(Flow Cytometric analysis of ATDC5 cells using MBS629248 (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-OTC antibody
OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
Product Categories/Family for anti-OTC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ornithine carbamoyltransferase
NCBI Official Symbol
OTC
NCBI Official Synonym Symbols
F1B16.13; F1B16_13; ornithine carbamoyltransferase; ORNITHINE CARBAMOYLTRANSFERASE; OTC
NCBI Protein Information
ornithine carbamoyltransferase
Protein Family
Similar Products
Product Notes
The OTC (Catalog #AAA629248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ornithine Carbamoyltransferase (OTC, Ornithine Transcarbamylase, OTCase, MGC129967, MGC129968, MGC138856, OCTD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ornithine Carbamoyltransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry. WB: 1:100-1:500 IHC (FFPE): 1:10-1:50 FC: 1:10-1:50. Researchers should empirically determine the suitability of the OTC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ornithine Carbamoyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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