Rabbit ORC3 Polyclonal Antibody | anti-ORC3 antibody

ORC3 Polyclonal Antibody

Cat. Num. AAA3007394

• Gene Names: ORC3; LAT; ORC3L; LATHEO
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ORC3; Polyclonal Antibody; ORC3 Polyclonal Antibody; Origin recognition complex subunit 3; Origin recognition complex subunit Latheo; LATHEO; ORC3L; anti-ORC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ORC3 polyclonal antibody detects endogenous levels of ORC3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 568

• Applicable Applications for anti-ORC3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 315-360 of Human ORC3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ORC3 polyclonal antibody at 1:1000 dilutionLane1:HEK293T whole cell lysate (56. 7ug) Lane2:HELA whole cell lysate (51. 3ug) Lane3:A549 whole cell lysate (57. 6ug) Lane4:H9C2 whole cell lysate (56. 1ug) Lane5:Raw264. 7 whole cell lysate (51ug))

Related Product Information for anti-ORC3 antibody

The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.

NCBI and Uniprot Product Information

• NCBI GI #: 308737003
• NCBI GeneID: 23595
• NCBI Accession #: NP_001184188.1
• NCBI GenBank Nucleotide #: NP_001184188.1
• UniProt Accession #: Q9UBD5
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: origin recognition complex subunit 3 isoform 3
• NCBI Official Synonym Full Names: origin recognition complex subunit 3
• NCBI Official Symbol: ORC3
• NCBI Official Synonym Symbols: LAT; ORC3L; LATHEO
• NCBI Protein Information: origin recognition complex subunit 3
• UniProt Protein Name: Origin recognition complex subunit 3
• Protein Family: Origin recognition complex
• UniProt Gene Name: ORC3
• UniProt Synonym Gene Names: LATHEO; ORC3L
• UniProt Entry Name: ORC3_HUMAN

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ORC3L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Belongs to the ORC3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q14.3-q16.1

Cellular Component: nucleoplasm; nuclear origin of replication recognition complex; origin recognition complex

Molecular Function: protein binding; DNA replication origin binding

Biological Process: mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle