Rabbit anti-Mouse, rat Olig1 Polyclonal Antibody | anti-OLIG1 antibody

Anti-Olig1

Cat. Num. AAA502173

• Gene Names: Olig1; Olg-1; Bhlhb6; Oligo1; bHLHe21; AI836478; AW494459
• Reactivity: Mouse, rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A purified (Protein A purified rabbit serum.)
• Synonyms: Olig1; Polyclonal Antibody; Anti-Olig1; anti-OLIG1 antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~27 kDa Olig1 protein in Western blots. The antibody also works well for immunohistochemistry, immunocytochemistry and immunoprecipitation.
• Purity/Purification: Protein A purified (Protein A purified rabbit serum.)
• Form/Format: Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. Contains no preservative.
• Sequence Length: 260

• Applicable Applications for anti-OLIG1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:3,000; IHC: 1:100; ICC: 1:100; IP: 1:50
• Antigen: Recombinant mouse Olig1.
• Immunogen Information: Recombinant mouse Olig1
• Immunogen Species: Mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in rat and mouse. Other species have not yet been tested.
• Biological Significance: Olig genes have been identified as the earliest known markers of oligodendrocyte lineage determination to date (Zhou et al., 2000). Olig1 is a transcription factor which promotes formation and maturation of oligodendrocytes, especially within the brain. It is expressed in the ventral spinal cord as early as 9.5 dpc and by 15.5 dpc, olig1 is dispersed throughout the gray matter. In the postnatal brain, it is present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Olig1 has been demonstrated as necessary in the repair of brain lesions in patients with multiple sclerosis (Arnett et al. 2004).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-OLIG1 antibody

Rabbit polyclonal antibody

References

• Zhou Q, Wang S, Anderson DJ (2000) Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 25(2):331-43
• Arnett, H.A., et al. (2004) bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS. Science 306(5704):2111-5.

NCBI and Uniprot Product Information

• NCBI GI #: 59858559
• NCBI GeneID: 50914
• NCBI Accession #: NP_058664.2
• NCBI GenBank Nucleotide #: NM_016968.4
• UniProt Accession #: Q9JKN5
• Molecular Weight: 27
• NCBI Official Full Name: oligodendrocyte transcription factor 1
• NCBI Official Synonym Full Names: oligodendrocyte transcription factor 1
• NCBI Official Symbol: Olig1
• NCBI Official Synonym Symbols: Olg-1; Bhlhb6; Oligo1; bHLHe21; AI836478; AW494459
• NCBI Protein Information: oligodendrocyte transcription factor 1; oligodendrocyte-specific bHLH transcription factor 1
• UniProt Protein Name: Oligodendrocyte transcription factor 1
• Protein Family: Oligodendrocyte transcription factor
• UniProt Gene Name: Olig1
• UniProt Synonym Gene Names: Oligo1
• UniProt Entry Name: OLIG1_MOUSE

Uniprot Description

Function: Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube. Ref.5

Subcellular location: Nucleus

By similarity.

Tissue specificity: Expressed specifically in brain. Ref.1

Developmental stage: Expressed in the ventral spinal cord as early as 9.5 dpc. Expression declines to undetectable levels by 10.5 dpc and reappears in a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the olfactory epithelium from 11.5dpc onward. Ref.3

Induction: By SHH. Ref.4

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Sequence caution: The sequence BAB18906.1 differs from that shown. Reason: Erroneous initiation.