Rabbit anti-Human, Mouse OLFML2B Polyclonal Antibody | anti-OLFML2B antibody

Rabbit Polyclonal (IgG) to Human OLFML2B

Cat. Num. AAA248408

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: OLFML2B; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human OLFML2B; Anti-OLFML2B Antibody (aa171-220) IHC-plus; DKFZP586L151; Photomedin-2; RP11-227F8.1; Olfactomedin-like 2B; Olfactomedin-like protein 2B; Human OLFML2B; anti-OLFML2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OLFML2B Antibody detects endogenous levels of total OLFML2B protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% Glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 750

• Applicable Applications for anti-OLFML2B antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (1:50); WB (1:500 - 1:1000); Peptide Enzyme-Linked Immunosorbent Assay (1:10000); optimal dilution to be determined by the researcher.; ; Usage Summary: Western Blot: Predicted MW 83 kDa
• Family/Subfamily: GPCR/Orapha-B
• Immunogen Species: OLFML2B antibody was raised against Human
• Immunogen: OLFML2B antibody was raised against synthetic peptide from human OLFML2B (aa171-220).
• Epitope: aa171-220
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-OLFML2B antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of extracts from HUVEC cells, using OLFML2B Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-OLFML2B antibody

G Protein-Coupled Receptor Ls195570 is an Orphan-B GPCR with an unknown ligand.

Product Categories/Family for anti-OLFML2B antibody

Family: GPCR; Subfamily: Orphan-B

NCBI and Uniprot Product Information

• NCBI GI #: 46195719
• NCBI GeneID: 25903
• NCBI Accession #: NP_056256.1
• NCBI GenBank Nucleotide #: NM_015441.2
• UniProt Accession #: Q68BL8; Q5VU96; Q6NX46; Q86X11; Q9Y3X6; B7ZA39
• NCBI Official Full Name: olfactomedin-like protein 2B isoform 2
• NCBI Official Synonym Full Names: olfactomedin-like 2B
• NCBI Official Symbol: OLFML2B
• NCBI Protein Information: olfactomedin-like protein 2B; photomedin-2
• UniProt Protein Name: Olfactomedin-like protein 2B
• Protein Family: Olfactomedin-like protein
• UniProt Gene Name: OLFML2B
• UniProt Entry Name: OLM2B_HUMAN

Uniprot Description

OLFML2B: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: extracellular region