Rabbit anti-Human OCT3/4 Polyclonal Antibody | anti-OCT3/4 antibody

OCT3/4 (C279) (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3) (PE)

Cat. Num. AAA6327567

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: OCT3/4; Polyclonal Antibody; OCT3/4 (C279) (POU5F1; OCT3; OCT4; OTF3; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3) (PE); POU5F1; Octamer-binding transcription factor 3; anti-OCT3/4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-OCT3/4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(OCT3/4 Antibody (C279) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the OCT3/4 antibody detected the OCT3/4 protein (arrow).)

Immunohistochemistry (IHC)

(OCT3/4 Antibody (C279) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OCT3/4 Antibody (C279) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-OCT3/4 antibody

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Product Categories/Family for anti-OCT3/4 antibody

Antibodies; Transcription Factors

References

Abu-Remaileh, M., et al. EMBO J. 29(19):3236-3248(2010). Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Schultz, S.S., et al. Mol. Cell. Biol. 30(18):4521-4534(2010). Kim, S., et al. Br. J. Cancer 102(2):436-446(2010). Wang, X., et al. Stem Cells 27(6):1265-1275(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 42560248
• NCBI GeneID: 5460
• NCBI Accession #: NP_002692.2
• NCBI GenBank Nucleotide #: NM_002701.4
• UniProt Accession #: Q01860; P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
• Molecular Weight: 38,571 Da
• NCBI Official Full Name: POU domain, class 5, transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; octamer-binding transcription factor-3; POU-type homeodomain-containing DNA-bi
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
• UniProt Entry Name: PO5F1_HUMAN

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]

Uniprot Description

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of asymmetric cell division; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter