Rabbit anti-Rat OATP3 (Organic Anion Transporting Polypeptide 3) Polyclonal Antibody | anti-OATP3 antibody

Rabbit anti-Rat OATP3 (Organic Anion Transporting Polypeptide 3)

Cat. Num. AAA613180

• Gene Names: Slco1a5; Oatp3; OATP-3; Slc21a7; Slco1a2
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: OATP3 (Organic Anion Transporting Polypeptide 3); Polyclonal Antibody; Rabbit anti-Rat OATP3 (Organic Anion Transporting Polypeptide 3); Anti -OATP3 (Organic Anion Transporting Polypeptide 3); anti-OATP3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Oatp3. No significant sequence homology with other Oatp2, Oatp3 or human OATP or LST-1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS. pH 7.2, 0.1% BSA
• Concentration: ~1 mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-OATP3 antibody: Suitable for use in ELISA (EIA), Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
• Application Notes: ELISA: 0.5-1 ug/mL Control peptide can be used to coat ELISA plates at 1 ug/ml.; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide consisting of 12aa sequence within the mid-region of rat Oatp3 conjugated with KLH. Species Sequence Homology: mouse Oatp3: 75%.
• Control peptide: OATP3, Rat Control Peptide (Organic Anion Transporting Polypeptide 3)
• Preparation and Storage: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-OATP3 antibody

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus). Oatp3 (670 aa; 12 TM), cloned from rat retina, shares ~80-83% identity with oatp1-2 and ~77% to rat OAT-K1. Oatp3 is highly expressed in the kidney, and moderately in the retina. Oatp3 mediates uptake of taurocholate, thyroxine, and triiodothyronine. Most of the T4 secreted from the thyroid is deiodinated in peripheral tissues. The liver and kidney are the major sites for the production of T3 and T4. Therefore, oatp3 may play a critical role in transporting thyroid hormones from the circulation to the deiodination sites in the kidney.

Product Categories/Family for anti-OATP3 antibody

Antibodies; Abs to Ion Channel

References

Naud, J.; J. Pharmacol. Exp. Ther. 320:978-985 (2007). 2. Abe, T., et al., J. Biol. Chem. 273: 22395-22401 (1998). 3. Noe, B., et al., PNAS 94: 10346-10350 (1997). 4. Abe, T., et al., J. Biol. Chem. 273: 22395-22401 (1998). 5. Kakyo, M., et al., FEBS Lett. 445: 343-346 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 3420183
• NCBI GeneID: 80900
• UniProt Accession #: O88397; Q9EQR8
• NCBI Official Full Name: organic anion transporter protein 3
• NCBI Official Synonym Full Names: solute carrier organic anion transporter family, member 1a5
• NCBI Official Symbol: Slco1a5
• NCBI Official Synonym Symbols: Oatp3; OATP-3; Slc21a7; Slco1a2
• NCBI Protein Information: solute carrier organic anion transporter family member 1A5; solute carrier family 21 member 7; organic anion transporting polypeptide 3; organic anion-transporting polypeptide 3; sodium-independent organic anion transporter 3; solute carrier family 21 (fatty acid transporter) member 7; solute carrier organic anion transporter family, member 1A2
• UniProt Protein Name: Solute carrier organic anion transporter family member 1A5
• UniProt Gene Name: Slco1a5
• UniProt Synonym Gene Names: Oatp1a5; Oatp3; Slc21a7; Slco1a2
• UniProt Entry Name: SO1A5_RAT

NCBI Description

facilitates renal uptake and excretion of the uremic toxin indoxyl sulfate; may facilitate intestinal absorption of bile acids; transports thyroid hormones and taurocholate [RGD]

Uniprot Description

Function: Mediates the Na+-independent transport of organic anions such as taurocholate and thyroid hormones.

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Highly expressed in the kidney and moderately abundant in the retina.

Sequence similarities: Belongs to the organo anion transporter (TC 2.A.60) family. [View classification]Contains 1 Kazal-like domain.