Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with OAT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).OAT immunoreactivity is localized to Mitochondrion significantly.)
Rabbit anti-Human OAT Polyclonal Antibody | anti-OAT antibody
OAT, NT (Ornithine Aminotransferase, Ornithine delta-Aminotransferase, Ornithine-oxo-acid Aminotransferase, DKFZp781A11155, GACR, HOGA, OATASE, OKT) (Azide free) (HRP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with OAT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).OAT immunoreactivity is localized to Mitochondrion significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with OAT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).OAT immunoreactivity is localized to Mitochondrion significantly.)
Western Blot (WB)
(Western blot analysis of OAT Antibody (N-term) in 293(lane 1), MDA-MB231 cell line(lane 2) lysates (35ug/lane). OAT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of OAT Antibody (N-term) in 293(lane 1), MDA-MB231 cell line(lane 2) lysates (35ug/lane). OAT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of OAT (arrow) using rabbit polyclonal OAT Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OAT gene.)
Western Blot (WB)
(Western blot analysis of OAT (arrow) using rabbit polyclonal OAT Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OAT gene.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]
Uniprot Description
OAT: Defects in OAT are the cause of hyperornithinemia with gyrate atrophy of choroid and retina (HOGA). HOGA is a slowly progressive blinding autosomal recessive disorder. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; Amino Acid Metabolism - arginine and proline; EC 2.6.1.13; Mitochondrial
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: ornithine-oxo-acid transaminase activity; pyridoxal phosphate binding
Biological Process: visual perception; amino acid biosynthetic process
Disease: Gyrate Atrophy Of Choroid And Retina
Research Articles on OAT
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Product Notes
The OAT oat (Catalog #AAA6490940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OAT, NT (Ornithine Aminotransferase, Ornithine delta-Aminotransferase, Ornithine-oxo-acid Aminotransferase, DKFZp781A11155, GACR, HOGA, OATASE, OKT) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the OAT oat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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