Rabbit anti-Human NUP35 Polyclonal Antibody | anti-NUP35 antibody

NUP35, CT (NUP35, MP44, NUP53, Nucleoporin NUP53, 35kD nucleoporin, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53, Nucleoporin Nup35) (FITC)

Cat. Num. AAA6327274

• Gene Names: NUP35; MP44; NP44; MP-44; NUP53
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUP35; Polyclonal Antibody; CT (NUP35; MP44; NUP53; Nucleoporin NUP53; 35kD nucleoporin; Mitotic phosphoprotein 44; Nuclear pore complex protein Nup53; Nucleoporin Nup35) (FITC); Nucleoporin Nup35; anti-NUP35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NUP35 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NUP35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-326 amino acids from the C-terminal region of human NUP35.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NUP35 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NUP35 antibody detected the NUP35 protein (arrow).)

Flow Cytometry (FC/FACS)

(NUP35 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NUP35 antibody

This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC.

Product Categories/Family for anti-NUP35 antibody

Antibodies; Nuclear Proteins

References

Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010). Hawryluk-Gara, L.A., et al. Mol. Biol. Cell 19(4):1753-1762(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 31982904
• NCBI GeneID: 129401
• NCBI Accession #: NP_612142.2
• NCBI GenBank Nucleotide #: NM_138285.4
• UniProt Accession #: Q8NFH5; Q4ZFZ9; Q53S95; Q8TDJ1; B4DP57; B4DYB4
• Molecular Weight: 21,142 Da
• NCBI Official Full Name: nucleoporin NUP53 isoform a
• NCBI Official Synonym Full Names: nucleoporin 35kDa
• NCBI Official Symbol: NUP35
• NCBI Official Synonym Symbols: MP44; NP44; MP-44; NUP53
• NCBI Protein Information: nucleoporin NUP53; mitotic phosphoprotein 44; nuclear pore complex protein Nup53
• UniProt Protein Name: Nucleoporin NUP53
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP35
• UniProt Synonym Gene Names: MP44; NUP53; MP-44
• UniProt Entry Name: NUP53_HUMAN

NCBI Description

This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]

Uniprot Description

NUP35: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. Belongs to the Nup53 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; nuclear membrane; intracellular membrane-bound organelle; plasma membrane; nuclear pore; nuclear envelope

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport