Western Blot (WB)
(Anti-NUDT5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:35 kDa)
Rabbit anti-Human NUDT5 Polyclonal Antibody | anti-NUDT5 antibody
Anti-NUDT5 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 0.5-2uL/mg of lysate
Western Blot (WB)
(Anti-NUDT5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:35 kDa)
Western Blot (WB)
(Anti-NUDT5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:35 kDa)
Immunoprecipitation (IP)
(NUDT5 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NUDT5 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(NUDT5 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-NUDT5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NUDT5 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 35 kDa)
Western Blot (WB)
(Anti-NUDT5 rabbit polyclonal antibody at 1:500 dilution Lane A: NUDT5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:35 kDa)
Western Blot (WB)
(Anti-NUDT5 rabbit polyclonal antibody at 1:500 dilution Lane A: NUDT5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:24 kDa Observed band size:35 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]
Uniprot Description
NUDT5: Hydrolyzes with similar activities ADP-ribose ADP- mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. Belongs to the Nudix hydrolase family.
Protein type: EC 3.6.1.13; Nucleotide Metabolism - purine; Phosphatase; EC 3.6.1.58
Chromosomal Location of Human Ortholog: 10p14
Cellular Component: intracellular; cytosol; nucleus
Molecular Function: snoRNA binding; m7G(5')pppN diphosphatase activity; ADP-ribose diphosphatase activity; nucleoside-diphosphatase activity; magnesium ion binding; ADP-sugar diphosphatase activity
Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; D-ribose catabolic process; ribonucleoside diphosphate catabolic process
Research Articles on NUDT5
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Product Notes
The NUDT5 nudt5 (Catalog #AAA8106690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUDT5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the NUDT5 nudt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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