Goat anti-Human NudE-L Polyclonal Antibody | anti-Ndel1 antibody

NudE-L (NudE-like Protein, Nudel, Nuclear Distribution Gene E Like Homolog 1, NDEL1, DKFZp451M0318, Endooligopeptidase A, EOPA, Mitosin-associated Protein 1, Mitosin-associated Protein MITAP1)

Cat. Num. AAA621597

• Gene Names: Ndel1; NUDEL; MITAP1; mNudel; 2600006O07Rik
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: NudE-L; Polyclonal Antibody; NudE-L (NudE-like Protein; Nudel; Nuclear Distribution Gene E Like Homolog 1; NDEL1; DKFZp451M0318; Endooligopeptidase A; EOPA; Mitosin-associated Protein 1; Mitosin-associated Protein MITAP1); Anti -NudE-L (NudE-like Protein; anti-Ndel1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NDEL1 at ~39kD. Species sequence homology: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Ndel1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:8000; Western Blot: 0.1-0.3ug/ml; Optimal dilutions to be determined by the researcher
• Immunogen: Synthetic peptide, CRNFAKDQASRKSY, from the internal region of human NDEL1.
• Positive Control: Human Brain (Cerebellum) lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 monthsat -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Ndel1 antibody

Nudel, or endooligopeptidase A, a protein highly expressed in the brain where it associates with dynein motors and Lis1, both of which have been linked to neuronal development and migration. Nudel facilitates the polymerisation of neurofilaments and the organisation of the cellular microtubule array and microtubule anchoring at the centrosome. It is required for several dynein- and microtubule-dependent processes such as the centripetal motion of secretory vesicles, coupling of the nucleus and centrosome and maintaining Golgi integrity. Nudel also serves as a Cdk5 substrate and its phosphorylation status affects its localisation in neurons.

Product Categories/Family for anti-Ndel1 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 238550188
• NCBI GeneID: 83431
• NCBI Accession #: NP_076157.2
• NCBI GenBank Nucleotide #: NM_023668.2
• UniProt Accession #: Q9ERR1; Q9D0Q4; Q9EPT6
• Molecular Weight: 38,366 Da
• NCBI Official Full Name: nuclear distribution protein nudE-like 1
• NCBI Official Synonym Full Names: nuclear distribution gene E-like homolog 1 (A. nidulans)
• NCBI Official Symbol: Ndel1
• NCBI Official Synonym Symbols: NUDEL; MITAP1; mNudel; 2600006O07Rik
• NCBI Protein Information: nuclear distribution protein nudE-like 1; mNudE-L; protein Nudel; OTTMUSP00000006155; OTTMUSP00000006156; OTTMUSP00000006157; nuclear disribution gene E like
• UniProt Protein Name: Nuclear distribution protein nudE-like 1
• Protein Family: Nuclear distribution protein
• UniProt Gene Name: Ndel1
• UniProt Synonym Gene Names: Nudel
• UniProt Entry Name: NDEL1_MOUSE

Uniprot Description

NDEL1: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Belongs to the nudE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Protease

Cellular Component: kinetochore; axon hillock; centrosome; kinesin complex; microtubule; microtubule associated complex; cytoskeleton; neurofilament cytoskeleton; axon; cytoplasm; leading edge; nucleolus; microtubule organizing center; nuclear envelope; chromosome; chromosome, pericentric region

Molecular Function: protein binding; microtubule binding; protein complex binding; beta-tubulin binding; alpha-tubulin binding

Biological Process: positive regulation of axon extension; multicellular organismal development; neuron migration; Wnt receptor signaling pathway through beta-catenin; proteolysis; retrograde axon cargo transport; chromosome segregation; mitotic centrosome separation; neurofilament cytoskeleton organization and biogenesis; establishment of mitotic spindle orientation; transport; vesicle transport along microtubule; cell differentiation; neurite development; nervous system development; positive regulation of axon regeneration; cell migration; central nervous system neuron axonogenesis; establishment of chromosome localization; nuclear envelope disassembly; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; inner cell mass cell proliferation; centrosome localization; cerebral cortex radially oriented cell migration