Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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Database
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Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
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⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of C...
$value['specificity']
This assay has high sensitivity and excellent specificity for detection of CPK. No significant cross-reactivity or interference between CPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPK and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value['purity']
⇄form => string (3) "N/A"
$value['form']
⇄concentration => string (3) "N/A"
$value['concentration']
⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
⇄⧉products_description => string (1318) "Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed f...
$value['products_description']
Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPK. This ELISA kit for research use only!<br><br>Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.
Human Creatine phosphokinase ELISA Kit | CPK elisa kit
datasheet
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Properties (10)
Available methods (45)
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$value->a
⇄products_id_mbs => string (6) "725147"
$value->a['products_id_mbs']
⇄products_id => string (5) "16248"
$value->a['products_id']
⇄products_quantity => string (4) "9999"
$value->a['products_quantity']
⇄products_model => string (8) "AAA16248"
$value->a['products_model']
⇄products_model_oem => string (8) "E01C0810"
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⇄ncbi_gi_num => string (3) "N/A"
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⇄ncbi_acc_num => string (3) "N/A"
$value->a['ncbi_acc_num']
⇄ncbi_acc_num_related => string (3) "N/A"
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⇄ncbi_sp_acc_num_related => string (3) "N/A"
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⇄ncbi_gb_acc_num => string (3) "N/A"
$value->a['ncbi_gb_acc_num']
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⇄products_image => string (9) "ELISA Kit"
$value->a['products_image']
⇄sequence_positions => string (3) "N/A"
$value->a['sequence_positions']
⇄sequence_length => string (3) "N/A"
$value->a['sequence_length']
⇄sequence => string (3) "N/A"
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⇄clonality => string (3) "N/A"
$value->a['clonality']
⇄isotype => string (3) "N/A"
$value->a['isotype']
⇄clone_number => string (3) "N/A"
$value->a['clone_number']
⇄host => string (3) "N/A"
$value->a['host']
⇄reactivity => string (5) "Human"
$value->a['reactivity']
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of C...
$value->a['specificity']
This assay has high sensitivity and excellent specificity for detection of CPK. No significant cross-reactivity or interference between CPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPK and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
⇄⧉products_description => string (1318) "Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed f...
$value->a['products_description']
Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPK. This ELISA kit for research use only!<br><br>Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of C...
$value->d['specificity']
This assay has high sensitivity and excellent specificity for detection of CPK. No significant cross-reactivity or interference between CPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPK and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
$value->d['form']
⇄concentration => string (3) "N/A"
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⇄storage_stability => string (34) "Store all reagents at 2-8 degree C"
⇄⧉products_description => string (1318) "Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed f...
$value->d['products_description']
Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPK. This ELISA kit for research use only!<br><br>Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.
⇄⧉products_description => string (1365) "Intended Uses: This CX3CR1 ELISA kit is a 1.5 hour solid-phase ELISA designe...
$value[0]['_source']['products_description']
Intended Uses: This CX3CR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CX3CR1. This ELISA kit for research use only!<br><br>Principle of the Assay||CX3CR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX3CR1 antibody and an CX3CR1-HRP conjugate. The assay sample and buffer are incubated together with CX3CR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX3CR1 concentration since CX3CR1 from samples and CX3CR1-HRP conjugate compete for the anti-CX3CR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX3CR1 from the sample, fewer sites are left to bind CX3CR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3CR1 concentration in each sample is interpolated from this standard curve.
⇄products_references => string (3) "N/A"
$value[0]['_source']['products_references']
⇄⧉products_related_diseases => string (209) "Inflammation||307!!Nervous System Diseases||181!!Disease Models, Animal||157...
$value[0]['_source']['products_related_diseases']
Inflammation||307!!Nervous System Diseases||181!!Disease Models, Animal||157!!Necrosis||107!!Atherosclerosis||92!!HIV Infections||50!!Kidney Diseases||40!!Coronary Disease||36!!Liver Diseases||31!!Fibrosis||24
<b>Storage:</b><br>Avoid repeated freeze/thaw cycles.<br>Store at 4 degree C for frequent use.<br>Aliquot and store at -20 degree C for 24 months.<br><br><b>Stability Test:</b><br>The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western blotting: 0.01-2ug/mL;<br>Immunohistochemistry: 5-20ug/mL;<br>Immunocytochemistry: 5-20ug/mL;<br>Optimal working dilutions must be determined by end user.
aaa20898 rabbit mus musculus mouse antigen specific affinity chromatography followed by protein a supplied as solution form in 0.01m pbs ph7.4 containing 0.05 proclin 300 50 glycerol western blot wb immunohistochemistry ihc immunocytochemistry icc immunoprecipitation ip blotting 0.5 2ug ml 5 20ug optimal working dilutions must be determined end user sample recombinant xcr1 aaa20898_wb dab staining on fromalin fixed paraffin embedded kidney tissue aaa20898_ihc antibody chemokine c motif receptor 1 polyclonal to xc gpr5 mxcr1 ccxcr1 lymphotactin scm1 12585214 q9r0m1 source preparation traits liquid cross reactivity rat immunogen thr244~tyr322 expressed e.coli mbs2029203 proclin300 blotting0.5 ml5 receptor1
⇄⧉products_description => string (828) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[2]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine CCL20 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of h...
$value[3]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human CCR2. No significant cross-reactivity or interference between human CCR2 and analogues was observed.
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[3]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Samples||Serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)!!Assay Type||Quantitative Sandwich!!Detection Range||23.44 pg/ml -1500 pg/ml!!Sensitivity||Typically less than 5.86 pg/ml.
⇄⧉etc_term2 => string (326) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[3]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[3]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄products_references => string (3) "N/A"
$value[3]['_source']['products_references']
⇄⧉products_related_diseases => string (229) "Inflammation||813!!Immune System Diseases||526!!Disease Models, Animal||359!...
$value[3]['_source']['products_related_diseases']
Inflammation||813!!Immune System Diseases||526!!Disease Models, Animal||359!!Cardiovascular Diseases||333!!Nervous System Diseases||291!!Necrosis||238!!Atherosclerosis||199!!Lung Diseases||167!!Fibrosis||115!!Kidney Diseases||106
⇄⧉search_terms => string (686) "aaa15001 human this assay has high sensitivity and excellent specificity for...
$value[3]['_source']['search_terms']
aaa15001 human this assay has high sensitivity and excellent specificity for detection of ccr2 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15001_td elisa kit chemokine c motif receptor 2 cc ckr ccr2a ccr2b cd192 ckr2 ckr2a ckr2b cmkbr2 mcp 1 r monocyte chemoattractant protein chemotactic type isoform a ccr 41,915 da cd_antigen ccr2_human 183979980 np_001116513.2 p41597 nm_001123041.2 q4vbl2 a0avq3 b2rmt0 gene 609423 samples serum plasma tissue homogenates cerebrospinal fluid csf quantitative sandwich range 23.44 pg ml 1500 typically less than 5.86 intra precision within an cv receptor2 mcp1
⇄⧉specificity => string (181) "This assay has high sensitivity and excellent specificity for detection of m...
$value[4]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of mouse CCR2. No significant cross-reactivity or interference between mouse CCR2 and analogues was observed.
⇄purity => string (3) "N/A"
$value[4]['_source']['purity']
⇄form => string (3) "N/A"
$value[4]['_source']['form']
⇄concentration => string (3) "N/A"
$value[4]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[4]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[4]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[4]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (722) "aaa18390 mouse this assay has high sensitivity and excellent specificity for...
$value[4]['_source']['search_terms']
aaa18390 mouse this assay has high sensitivity and excellent specificity for detection of ccr2 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18390_td elisa kit chemokine c motif receptor 2 type cc ckr ccr2a ccr2b cd192 ckr2 ckr2a ckr2b cmkbr2 mcp 1 r monocyte chemoattractant protein chemotactic mje ccr mip alphar je fic 42,783 da cd_antigen ccr2_mouse 6753466 np_034045.1 p51683 nm_009915.2 q61172 samples serum plasma tissue homogenates quantitative sandwich range 15.6 pg ml 1000 < 3.9 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in receptor2 mcp1 <3.9
⇄⧉products_description => string (829) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[5]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine CXCL1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄products_references => string (3) "N/A"
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⇄products_related_diseases => string (3) "N/A"
$value[5]['_source']['products_related_diseases']
⇄products_categories => string (3) "N/A"
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⇄ncbi_full_name => string (3) "N/A"
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⇄ncbi_full_name_syn => string (3) "N/A"
$value[5]['_source']['ncbi_full_name_syn']
⇄ncbi_symbol => string (3) "N/A"
$value[5]['_source']['ncbi_symbol']
⇄ncbi_symbol_syn => string (3) "N/A"
$value[5]['_source']['ncbi_symbol_syn']
⇄ncbi_protein_info => string (3) "N/A"
$value[5]['_source']['ncbi_protein_info']
⇄ncbi_chrom_loc => string (3) "N/A"
$value[5]['_source']['ncbi_chrom_loc']
⇄ncbi_gene_id => string (3) "N/A"
$value[5]['_source']['ncbi_gene_id']
⇄ncbi_mol_weight => string (3) "N/A"
$value[5]['_source']['ncbi_mol_weight']
⇄ncbi_pathways => string (3) "N/A"
$value[5]['_source']['ncbi_pathways']
⇄sp_protein_name => string (3) "N/A"
$value[5]['_source']['sp_protein_name']
⇄sp_protein_name_syn => string (3) "N/A"
$value[5]['_source']['sp_protein_name_syn']
⇄sp_gene_name => string (3) "N/A"
$value[5]['_source']['sp_gene_name']
⇄sp_gene_name_syn => string (3) "N/A"
$value[5]['_source']['sp_gene_name_syn']
⇄sp_entry_name => string (3) "N/A"
$value[5]['_source']['sp_entry_name']
⇄sp_mim => string (3) "N/A"
$value[5]['_source']['sp_mim']
⇄sp_interactions => string (3) "N/A"
$value[5]['_source']['sp_interactions']
⇄products_url => string (3) "N/A"
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⇄products_viewed => string (1) "0"
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⇄⧉search_terms => string (448) "aaa22557 porcine no cross reaction with other factors typical testing data s...
$value[5]['_source']['search_terms']
aaa22557 porcine no cross reaction with other factors typical testing data standard curve for reference only aaa22557_sc elisa kit chemokine c x motif ligand 1 melanoma growth stimulating activity alpha cxcl1 samples serum plasma or cell culture supernatant and organizations in the natural recombinant ctx i concentration assay type sandwich detection range 10 ng ml 0.156 sensitivity 0.05 intra precision <= 8 inter 12 ligand1 range10 <=8 inter12
⇄⧉sequence => string (369) "<font color="red">Antigen: </font>The target protein is fused with N-termina...
$value[6]['_source']['sequence']
<font color="red">Antigen: </font>The target protein is fused with N-terminal His-Tag and its sequence is listed below.<br>MGHHHHHHSG SEF- WVKDAMQHLD RQAAALTRNG GTFEKQIGEV KPRTTPAAGG MDESVVLEPE ATGESSSLEP TPSSQEAQRA LGTSPELPTG VTGSSGTRLP PTPKAQDGGP VGTELFRVPP VSTAATWQSS APHQPGPSLW AEAKTSEAPS TQDPSTQAST ASSPAPEENA PSEGQRVWGQ GQSPRPENSL EREEMGPVPA HTDAFQDWGP GSMAHVSVVP
⇄clonality => string (10) "Polyclonal"
$value[6]['_source']['clonality']
⇄isotype => string (3) "N/A"
$value[6]['_source']['isotype']
⇄clone_number => string (3) "N/A"
$value[6]['_source']['clone_number']
⇄host => string (6) "Rabbit"
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⇄reactivity => string (5) "Human"
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The antibody is a rabbit polyclonal antibody raised against CX3CL1. It has been selected for its ability to recognize CX3CL1 in immunohistochemical staining andwestern blotting.
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Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western blotting: 1:100-400<br>Immunocytochemistry in formalin fixed cells: 1:100-500<br>Immunohistochemistry in formalin fixed frozen section: 1:100-500<br>Immunohistochemistry in paraffin section: 1:50-200<br>Enzyme-linked Immunosorbent Assay: 1:100-200
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IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.||AAA20087_IHC8.jpg!!IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Breast Cancer Tissue||AAA20087_IHC7.jpg!!IHC (Immunohistchemistry)||DAB staining on IHC-P; Samples: Human Liver Tissue||AAA20087_IHC6.jpg!!IHC (Immunohistochemistry)||HE staining on IHC-P; Samples: Human Testis Tissue||AAA20087_IHC5.jpg!!IHC (Immunohistochemistry)||HE staining on IHC-P; Samples: Human Kidney Tissue||AAA20087_IHC4.jpg!!IHC (Immunohistochemistry)||DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.||AAA20087_IHC3.jpg!!WB (Western Blot)||Western Blot: Sample: Recombinant CX3CL1, Human.||AAA20087_WB2.jpg!!WB (Western Blot)||Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue.||AAA20087_WB.jpg
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aaa20087 rabbit human polyclonal affinity chromatography supplied as solution form in pbs ph7.4 containing 0.02 nan3,50 glycerol the antibody is a raised against cx3cl1 it has been selected for its ability to recognize immunohistochemical staining andwestern blotting immunocytochemistry icc immunohistochemistry ihc formalin paraffin elisa eia western blot wb 1:100 400 fixed cells 500 frozen section 1:50 200 enzyme linked immunosorbent assay sample lane1 mouse brain tissue lane2 heart aaa20087_wb recombinant aaa20087_wb2 dab on p samples stomach cancer aaa20087_ihc he kidney aaa20087_ihc2 testis aaa20087_ihc3 liver aaa20087_ihc4 breast aaa20087_ihc5 prostate gland aaa20087_ihc6 antigen target protein fused with n terminal his tag and sequence listed below mghhhhhhsg sef wvkdamqhld rqaaaltrng gtfekqigev kprttpaagg mdesvvlepe atgessslep tpssqeaqra lgtspelptg vtgssgtrlp ptpkaqdggp vgtelfrvpp vstaatwqss aphqpgpslw aeaktseaps tqdpstqast asspapeena psegqrvwgq gqsprpensl ereemgpvpa htdafqdwgp gsmahvsvvp chemokine c x3 motif ligand 1 fractalkine isoform ntn ntt cxc3 cxc3c scyd1 abcd 3 c3xkine neurotactin 42,203 da cx3c membrane anchored small inducible cytokine d1 fkn 4506857 np_002987.1 p78423 nm_002996.5 o00672 u91835 mrna immunogen trp81~ala336 expressed e.coli cross reactivity conjugated apc version of this item also available catalog #mbs2035742 1:100400 cells500 1:50200 ligand1 abcd3
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse CXCL2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
<a href="https://www.nature.com/articles/s41598-018-30087-4" target="_blank" rel="nofollow">Antenatal IL-1-dependent inflammation persists postnatally and causes retinal and sub-retinal vasculopathy in progeny</a><br><a href="http://www.jimmunol.org/content/early/2017/02/01/jimmunol.1601600" target="_blank" rel="nofollow">Antenatal suppression of IL-1 protects against inflammation-induced fetal injury and improves neonatal and developmental outcomes in mice
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Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Competitive!!Detection Range||0.5-10ng/mL!!Sensitivity||0.1ng/mL
⇄⧉etc_term2 => string (199) "Intended Uses||This CXCR4 ELISA kit is a 1.5 hour solid-phase ELISA designed...
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Intended Uses||This CXCR4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CXCR4. This ELISA kit for research use only, not for therapeutic applications!
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⇄products_name => string (32) "Chemokine C X C Motif Receptor 4"
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⇄products_name_oem => string (48) "Human Chemokine C X C Motif Receptor 4 ELISA Kit"
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⇄products_gene_name => string (5) "CXCR4"
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⇄⧉products_description => string (1187) "<b>Princple of the assay: </b>CXCR4 ELISA kit applies the competitive enzyme...
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<b>Princple of the assay: </b>CXCR4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR4 antibody and an CXCR4-HRP conjugate. The assay sample and buffer are incubated together with CXCR4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR4 concentration since CXCR4 from samples and CXCR4-HRP conjugate compete for the anti-CXCR4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR4 from the sample, fewer sites are left to bind CXCR4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR4 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (496) "aaa16350 human typical testing data standard curve for reference only aaa163...
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aaa16350 human typical testing data standard curve for reference only aaa16350_td elisa kit chemokine c x motif receptor 4 cxcr4 cd1 loc443442 57164257 np_001009425.1 nm_001009425.1 immunology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type competitive detection range 0.5 10ng ml sensitivity 0.1ng intended uses this is a 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications! receptor4 range0.5 a1.5
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human CXCR4. No significant cross-reactivity or interference between human CXCR4 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (836) "aaa15256 human this assay has high sensitivity and excellent specificity for...
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aaa15256 human this assay has high sensitivity and excellent specificity for detection of cxcr4 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15256_td elisa kit chemokine c x motif receptor 4 cxc cd184 d2s201e fb22 hm89 hsy3rr lap3 lcr1 lestr npy3r npyr npyrl npyy3r whim type antigen fusin leukocyte derived seven transmembrane domain lip isoform a lap 3 sdf 1 lps associated protein neuropeptide y y3 helix stromal cell factor lipopolysaccharide segment spleen 39,746 da cd_antigen r4 cxcr cxcr4_human 56790927 np_001008540.1 p61073 nm_001008540.1 o60835 p30991 p56438 q53s69 q9bxa0 q9ukn2 b2r5n0 gene 193670 samples serum plasma tissue homogenates quantitative sandwich range 15.6 pg ml 1000 < 3.9 intra precision within an cv receptor4 sdf1 <3.9
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human CCL16. No significant cross-reactivity or interference between human CCL16 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉ncbi_protein_info => string (293) "C-C motif chemokine 16; monotactin-1; chemokine LEC; chemokine CC-4; new CC ...
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C-C motif chemokine 16; monotactin-1; chemokine LEC; chemokine CC-4; new CC chemokine 4; liver CC chemokine-1; IL-10-inducible chemokine; liver-expressed chemokine; small-inducible cytokine A16; lymphocyte and monocyte chemoattractant; small inducible cytokine subfamily A (Cys-Cys), member 16
⇄⧉search_terms => string (639) "aaa18250 human this assay has high sensitivity and excellent specificity for...
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aaa18250 human this assay has high sensitivity and excellent specificity for detection of ccl16 no significant cross reactivity or interference between analogues was observed elisa kit chemokine c motif ligand 16 ckb12 hcc 4 ilinck lcc 1 lec lmc mgc117051 mtn ncc ncc4 scya16 scyl4 il 10 inducible cc liver expressed lymphocyte monotactin new small cytokine a16 monocyte chemoattractant subfamily a cys member 13,600 da ccl16_human 4759074 np_004581.1 o15467 nm_004590.2 q4kku0 601394 samples serum plasma tissue homogenates type sandwich range 39.06 pg ml 2500 typically less than 9.77 intra precision within an cv ligand16 hcc4 lcc1 il10
⇄⧉specificity => string (169) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CCR6. No significant cross-reactivity or interference between CCR6 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
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Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉etc_term1 => string (152) "Assay Type||Sandwich!!Samples||Serum, plasma, tissue homogenates and other b...
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Assay Type||Sandwich!!Samples||Serum, plasma, tissue homogenates and other biological fluids!!Detection Range||15.625-1000pg/ml!!Sensitivity||9.375pg/ml
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
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Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄⧉search_terms => string (704) "aaa17304 human this assay has high sensitivity and excellent specificity for...
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aaa17304 human this assay has high sensitivity and excellent specificity for detection of ccr6 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17304_sc elisa kit c chemokine receptor type 6 cd196 gpr29 gpr cy4 strl22 bn 1 cc ckr ccr antigen motif l3g protein coupled 29 ckrl3larc cmkbr6 dry6 dry lymphocyte 22 dcr2 ckrl3 l3 gprcy4 42,494 da like 3 g larc cd_antigen ccr6_human 37188165 np_113597.2 p51684 nm_031409.3 p78553 q92846 e1p5c6 601835 samples serum plasma tissue homogenates other biological fluids quantitative sandwich range 15.625 1000pg ml 9.375pg intra precision cv type6 bn1 coupled29 lymphocyte22 like3
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of B...
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This assay has high sensitivity and excellent specificity for detection of BKEC. No significant cross-reactivity or interference between BKEC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BKEC and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
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⇄products_name => string (37) "Breast And Kidney Expressed Chemokine"
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⇄products_name_oem => string (54) "Canine Breast And Kidney Expressed Chemokine ELISA Kit"
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⇄products_gene_name => string (4) "BRAK"
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⇄⧉products_description => string (1390) "Principle of the Assay: BKEC ELISA kit applies the competitive enzyme immuno...
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Principle of the Assay: BKEC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BKEC antibody and an BKEC-HRP conjugate. The assay sample and buffer are incubated together with BKEC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BKEC concentration since BKEC from samples and BKEC-HRP conjugate compete for the anti-BKEC antibody binding site. Since the number of sites is limited, as more sites are occupied by BKEC from the sample, fewer sites are left to bind BKEC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BKEC concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This BKEC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BKEC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa17201 canine elisa kit breast and kidney expressed chemokine brak c x motif ligand 14 cxcl14 11,730 da protein q8k453_rat 21667448 aam74057.1 d4a5s7 samples serum plasma cell culture supernatants body fluid tissue homogenate assay type competitive detection range 0.5 10ng ml sensitivity 0.1ng ligand14 range0.5
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of mouse MCP-3. No significant cross-reactivity or interference between mouse MCP-3 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Mouse monocyte chemotactic protein 3; MCP-3 ELISA kit; FIC; MARC; MCP-3; MCP3; MGC138463; MGC138465; NC28; SCYA6; SCYA7; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3) ; chemokine (C-C motif) ligand 7
⇄products_gene_name => string (4) "CCL7"
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⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (728) "aaa15219 mouse this assay has high sensitivity and excellent specificity for...
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aaa15219 mouse this assay has high sensitivity and excellent specificity for detection of mcp 3 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15219_td elisa kit chemokine c motif ligand 7 monocyte chemotactic protein fic marc mcp3 mgc138463 mgc138465 nc28 scya6 scya7 chemoattractant small inducible cytokine a7 ccl7 rantes sis homolog intercrine 10,999 da ccl7_mouse 7305463 np_038682.1 q03366 nm_013654.3 samples serum plasma tissue homogenates type quantitative sandwich range 1.56 pg ml 100 < 0.39 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ligand7 ml100
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CCR3. No significant cross-reactivity or interference between CCR3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCR3 and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
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⇄products_name => string (36) "C C chemokine receptor type 3 (CCR3)"
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⇄products_name_oem => string (52) "Human C C chemokine receptor type 3 (CCR3) ELISA Kit"
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⇄⧉products_description => string (1389) "Principle of the Assay: CCR3 ELISA kit applies the competitive enzyme immuno...
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Principle of the Assay: CCR3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCR3 antibody and an CCR3-HRP conjugate. The assay sample and buffer are incubated together with CCR3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCR3 concentration since CCR3 from samples and CCR3-HRP conjugate compete for the anti-CCR3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCR3 from the sample, fewer sites are left to bind CCR3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCR3 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This CCR3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCR3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human CXCL17. No significant cross-reactivity or interference between human CXCL17 and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (739) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (620) "aaa18141 human this assay has high sensitivity and excellent specificity for...
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aaa18141 human this assay has high sensitivity and excellent specificity for detection of cxcl17 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18141_td elisa kit chemokine c x motif ligand 17 dmc dcip1 mgc138300 unq473 vcc 1 vcc1 vegf co regulated coregulated dendritic cell monocyte like protein 13,819 da pro842 vcc1_human 85567182 aai12096.1 q6uxb2 a8kac0 611387 samples serum plasma culture supernates urine tissue homogenates type quantitative sandwich range 125 pg ml 8000 < 31.25 intra precision within an cv ligand17 range125
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Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of monkey CCL17. No significant cross-reactivity or interference between monkey CCL17 and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Monkey C-C motif chemokine 17 (CCL17) ELISA kit; A-152E5.3; ABCD-2; MGC138271; MGC138273; SCYA17; TARC; T cell-directed CC chemokine; small inducible cytokine A17; small inducible cytokine subfamily A (Cys-Cys); member 17; thymus and activation-regul; chemokine (C-C motif) ligand 17
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (737) "aaa18087 monkey this assay has high sensitivity and excellent specificity fo...
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aaa18087 monkey this assay has high sensitivity and excellent specificity for detection of ccl17 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18087_td elisa kit chemokine c motif ligand 17 a 152e5.3 abcd 2 mgc138271 mgc138273 scya17 tarc t cell directed cc small inducible cytokine a17 subfamily cys member thymus activation regul regulated 10,549 da ccl17_macmu 74136265 np_001028024.1 q8hyp9 nm_001032852.1 samples serum plasma tissue homogenates type quantitative sandwich range 39 pg ml 2500 <9.8 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ligand17 abcd2 range39
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of mouse MCP-2. No significant cross-reactivity or interference between mouse MCP-2 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Mouse monocyte chemotactic protein 2; MCP-2 ELISA kit; HC14; MCP-2; MCP2; SCYA10; SCYA8; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys); member 8 (monocyte che; chemokine (C-C motif) ligand 8
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⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (721) "aaa15171 mouse this assay has high sensitivity and excellent specificity for...
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aaa15171 mouse this assay has high sensitivity and excellent specificity for detection of mcp 2 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15171_td elisa kit chemokine c motif ligand 8 monocyte chemotactic protein hc14 mcp2 scya10 scya8 chemoattractant small inducible cytokine a8 subfamily a cys member che ccl8 ab023418 1810063b20rik 11,017 da ccl8_mouse 10946818 np_067418.1 q9z121 nm_021443.3 samples serum plasma tissue homogenates type quantitative sandwich range 6.25 pg ml 400 < 1.56 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ligand8 ml400
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CMKLR1. No significant cross-reactivity or interference between CMKLR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CMKLR1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1415) "Principle of the Assay: CMKLR1 ELISA kit applies the competitive enzyme immu...
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Principle of the Assay: CMKLR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CMKLR1 antibody and an CMKLR1-HRP conjugate. The assay sample and buffer are incubated together with CMKLR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CMKLR1 concentration since CMKLR1 from samples and CMKLR1-HRP conjugate compete for the anti-CMKLR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CMKLR1 from the sample, fewer sites are left to bind CMKLR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CMKLR1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This CMKLR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CMKLR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (471) "aaa27250 mouse typical testing data standard curve for reference only aaa272...
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aaa27250 mouse typical testing data standard curve for reference only aaa27250_td elisa kit chemokine like receptor 1 cmklr1 dez rver1 chemr23 chemerinr chemerin resolvin e1 g protein coupled orphan 42,035 da cml1_human 40794511 aar90850.1 q99788 o75748 q3kp37 q5u0h0 q99789 a8k6y5 602351 signal transduction samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative competitive sensitivity 0.1 ng ml receptor1 sensitivity0.1
