Rabbit anti-Human Nucleoside Diphosphate Kinase 7 Polyclonal Antibody | anti-NME7 antibody

Nucleoside Diphosphate Kinase 7, NT (NDP Kinase 7, NDK 7, FLJ37194, nm23-H7, NME7)

Cat. Num. AAA623809

• Gene Names: NME7; MN23H7; nm23-H7; FLJ37194
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Nucleoside Diphosphate Kinase 7; Polyclonal Antibody; NT (NDP Kinase 7; NDK 7; FLJ37194; nm23-H7; NME7); Anti -Nucleoside Diphosphate Kinase 7; anti-NME7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NME7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-NME7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human NME7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of NME7 (arrow) using rabbit polyclonal NME7 Antibody (V40) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NME7 gene.)

Western Blot (WB)

(NME7 Antibody (V40) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the NME7 antibody detected the NME7 protein (arrow).)

Related Product Information for anti-NME7 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-NME7 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 37574614
• NCBI GeneID: 29922
• NCBI Accession #: NP_932076.1
• NCBI GenBank Nucleotide #: NM_197972.1
• UniProt Accession #: Q59GR0; Q9Y5B8
• Molecular Weight: 32,240 Da
• NCBI Official Full Name: nucleoside diphosphate kinase 7 isoform b
• NCBI Official Synonym Full Names: non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)
• NCBI Official Symbol: NME7
• NCBI Official Synonym Symbols: MN23H7; nm23-H7; FLJ37194
• NCBI Protein Information: nucleoside diphosphate kinase 7; NDK 7; NDP kinase 7; OTTHUMP00000032536; OTTHUMP00000231077; OTTHUMP00000231078; nucleoside-diphosphate kinase 7
• UniProt Protein Name: Nucleoside-diphosphate kinase 7 isoform a variant
• Protein Family: Nucleoside diphosphate kinase
• UniProt Entry Name: Q59GR0_HUMAN

Uniprot Description

NDK7: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.6; Kinase, other; Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: centrosome

Molecular Function: metal ion binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: GTP biosynthetic process; CTP biosynthetic process; UTP biosynthetic process; intraflagellar transport; nucleoside diphosphate phosphorylation; brain development; determination of left/right symmetry