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Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 7.5 % SDS PAGE gel and Nucleoporin p62 antibody at a dilution of 1:5000)
Rabbit Nucleoporin p62 Polyclonal Antibody
Nucleoporin p62 antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Nucleoporin p62 antibody was purified by antigen-affinity chromatography
Synonyms
Nucleoporin p62; Polyclonal Antibody; Nucleoporin p62 antibody; Polyclonal Nucleoporin p62; Anti-Nucleoporin p62; 62 kDa nucleoporin; nucleoporin Nup62; p62; FLJ20822; Nucleoporin p 62; FLJ43869; NUP62; IBSN; Nucleoporin p-62; SNDI; MGC841; DKFZp547L134; anti-Nucleoporin p62 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Nucleoporin p62 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Nucleoporin p62 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:1000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:1000-1:10000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:1000-1:10000
Biological Significance
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals.
Cross-Reactivity
Human
Immunogen
Nucleoporin p62 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 230 and 490 of Nucleoporin p62
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 7.5 % SDS PAGE gel and Nucleoporin p62 antibody at a dilution of 1:5000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 7.5 % SDS PAGE gel and Nucleoporin p62 antibody at a dilution of 1:5000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:Hep G2; B:MOLT4; C:Raji) using a 10 % SDS PAGE gel and Nucleoporin p62 antibody at a dilution of 1:5000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:Hep G2; B:MOLT4; C:Raji) using a 10 % SDS PAGE gel and Nucleoporin p62 antibody at a dilution of 1:5000)
Immunofluorescence (IF)
(Nucleoporin p62 antibody detects NUP62 protein at Cytoplasm and Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUP62 protein stained by Nucleoporin p62 antibody diluted at 1:500. Blue: Hoechst 33343 staining.)
Immunofluorescence (IF)
(Nucleoporin p62 antibody detects NUP62 protein at Cytoplasm and Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUP62 protein stained by Nucleoporin p62 antibody diluted at 1:500. Blue: Hoechst 33343 staining.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using Nucleoporin p62 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using Nucleoporin p62 antibody at a dilution of 1:500)
Related Product Information for anti-Nucleoporin p62 antibody
Affinity purified Rabbit polyclonal Nucleoporin p62 antibody
Product Categories/Family for anti-Nucleoporin p62 antibody
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Product Notes
The Nucleoporin p62 (Catalog #AAA835314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nucleoporin p62 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:1000 ICC:1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:1000-1:10000. Researchers should empirically determine the suitability of the Nucleoporin p62 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoporin p62, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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