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Western Blot (WB) (Western Blot: Sample: Lane1: Human 293T Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)

Rabbit anti-Human Nucleoporin 155kDa Polyclonal Antibody | anti-NUP155 antibody

Nucleoporin 155kDa (NUP155) Polyclonal Antibody

Gene Names
NUP155; N155; ATFB15
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Nucleoporin 155kDa; Polyclonal Antibody; Nucleoporin 155kDa (NUP155) Polyclonal Antibody; N155; anti-NUP155 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NUP155. It has been selected for its ability to recognize NUP155 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-NUP155 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
NUP155 (Gln1154~Asn1379)
Quality Control
Content: The quality control contains recombinant NUP155 (Gln1154~Asn1379) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052011
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human 293T Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human 293T Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant NUP155, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant NUP155, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
149,016 Da
NCBI Official Full Name
nuclear pore complex protein Nup155 isoform 2
NCBI Official Synonym Full Names
nucleoporin 155
NCBI Official Symbol
NUP155
NCBI Official Synonym Symbols
N155; ATFB15
NCBI Protein Information
nuclear pore complex protein Nup155
UniProt Protein Name
Nuclear pore complex protein Nup155
UniProt Gene Name
NUP155
UniProt Synonym Gene Names
KIAA0791

NCBI Description

Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6. [provided by RefSeq, May 2013]

Uniprot Description

NUP155: Essential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport. Belongs to the non-repetitive/WGA-negative nucleoporin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 5p13.2

Cellular Component: membrane; nuclear envelope; nuclear membrane

Molecular Function: protein binding; structural constituent of nuclear pore; transporter activity

Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; nuclear envelope organization; protein import into nucleus; protein sumoylation; regulation of glycolysis; RNA export from nucleus; tRNA export from nucleus; viral process; viral transcription

Disease: Atrial Fibrillation, Familial, 15

Research Articles on NUP155

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Product Notes

The NUP155 nup155 (Catalog #AAA2032826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nucleoporin 155kDa (NUP155) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoporin 155kDa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NUP155 nup155 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoporin 155kDa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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