Rabbit anti-Human, Rat Nucleolin Polyclonal Antibody | anti-NCL antibody

Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)

Cat. Num. AAA611946

• Gene Names: NCL; C23; FLJ45706; FLJ59041
• Reactivity: Human, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Serum; Whole antisera
• Synonyms: Nucleolin; Polyclonal Antibody; Nucleolin (NCL; Nucl; C23; FLJ45706; Protein C23); Anti -Nucleolin (NCL; anti-NCL antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Detect human and rat nucleolin proteins.
• Purity/Purification: Serum; Whole antisera

• Applicable Applications for anti-NCL antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Immunogen: A 15 residue peptide designed to from C-terminus of the human protein.
• Cellular Localization: Nucleoli
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NCL antibody

Nucleolin is an abundant, 106kD nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker.

Product Categories/Family for anti-NCL antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 55956788
• NCBI GeneID: 4691
• NCBI Accession #: NP_005372.2
• NCBI GenBank Nucleotide #: NM_005381.2
• UniProt Accession #: P19338; Q53SK1; Q8NB06; Q9UCF0; Q9UDG1; Q6ZS99; Q9BQ02
• Molecular Weight: 76,614 Da
• NCBI Official Full Name: nucleolin
• NCBI Official Synonym Full Names: nucleolin
• NCBI Official Symbol: NCL
• NCBI Official Synonym Symbols: C23; FLJ45706; FLJ59041
• NCBI Protein Information: nucleolin; OTTHUMP00000204507
• UniProt Protein Name: Nucleolin
• Protein Family: Nucleolin
• UniProt Gene Name: NCL
• UniProt Entry Name: NUCL_HUMAN

NCBI Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq]

Uniprot Description

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Nuclear receptor co-regulator; Helicase; DNA-binding; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; ribonucleoprotein complex; nucleus

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: angiogenesis